Abstract

This Research Project on Lung Maturation will focus on a number of functional and biochemical markers of lung maturation in animals provided to this project by the Preterm Lamb Core. These animals will have been treated by ultrasound injections as fetuses with hormones given as single doses and as combinations (corticosteroid, T3, TRH and EGF) to previously unmanipulated fetal sheep. The goals of this project are to characterize the pulmonary responses by evaluating postnatal lung function as assessed by sequential measurements of gas exchange, lung compliances, lung volumes (functional residual capacity and lung volumes at 40 cmH2O pressures) and responses to exogenous surfactant over the first five hours after preterm delivery. Subsequently, alveolar surfactant saturated phosphatidylcholine and SP-A pool sizes will be measured as will antioxidant enzyme levels and SP-A, SP-B, and SP-C in mRNA levels in lung tissue and type II cells isolated from the tissue. These multiple endpoints will reflect both functional and biochemical assessments of the induced lung maturation responses in preterm lambs exposed to short duration, clinically relevant hormone treatment strategies.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Specialized Center (P50)
Project #
5P50HD029713-02
Application #
3757397
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
1994
Total Cost
Indirect Cost

  Institution

Name
La Biomed Research Institute/ Harbor UCLA Medical Center
Department
Type
DUNS #
City
Torrance
State
CA
Country
United States
Zip Code
90502

  Publications

Smith, Lynne M; Ervin, M Gore; Wada, Norihisa et al. (2003) Single and multiple prenatal glucocorticoid exposures improve preterm newborn lamb cardiovascular and renal function similarly. Am J Obstet Gynecol 188:444-53
Ervin, M G; Padbury, J F; Polk, D H et al. (2000) Antenatal glucocorticoids alter premature newborn lamb neuroendocrine and endocrine responses to hypoxia. Am J Physiol Regul Integr Comp Physiol 279:R830-8
Ibe, B O; Sander, F C; Raj, J U (2000) Platelet-activating factor receptors in lamb lungs are downregulated immediately after birth. Am J Physiol Heart Circ Physiol 278:H1168-76
Smith, L M; Ervin, M G; Wada, N et al. (2000) Antenatal glucocorticoids alter postnatal preterm lamb renal and cardiovascular responses to intravascular volume expansion. Pediatr Res 47:622-7
Ibe, B O; Sander, F C; Raj, J U (2000) Platelet activating factor acetylhydrolase activity in lamb lungs is up-regulated in the immediate newborn period. Mol Genet Metab 69:46-55
Tan, R C; Ikegami, M; Jobe, A H et al. (1999) Developmental and glucocorticoid regulation of surfactant protein mRNAs in preterm lambs. Am J Physiol 277:L1142-8
Gao, Y; Tolsa, J F; Shen, H et al. (1998) A single dose of antenatal betamethasone enhances isoprenaline and prostaglandin E2-induced relaxation of preterm ovine pulmonary arteries. Biol Neonate 73:182-9
Jobe, A H; Wada, N; Berry, L M et al. (1998) Single and repetitive maternal glucocorticoid exposures reduce fetal growth in sheep. Am J Obstet Gynecol 178:880-5
Okogbule-Wonodi, A C; Ibe, B O; Yue, B W et al. (1998) Phosphodiesterase activity in intrapulmonary arteries and veins of perinatal lambs. Mol Genet Metab 65:229-37
Emerson, G A; Bry, K; Hallman, M et al. (1997) Intra-amniotic interleukin-1 alpha treatment alters postnatal adaptation in premature lambs. Biol Neonate 72:370-9

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