Abstract

A YAC-based overlapping clone/STS/genetic linkage map of chromosome 7 will be constructed. Primer pairs will be developed from the sequence of 200 to 1000 bp clones of chromosome specific DNA, obtained from flow-sorted chromosomes, and will be used in PCR reactions to screen a library of human DNA in YACs for cognate clones. Overlapping YACs will be used to obtain contigs of an average length at least 2 Mb aligned by their probe content. Subsidiary efforts will be made to identify YACs and build contigs from existing probes in regions of current interest on chromosome 7 (e.g., cloned genes). Closure of the map, and its merger with genetic and cytogenetic data, will be based first on the generation of clones from the ends of YAC contigs, and identification of polymorphisms in these regions of DNA. End-clone polymorphisms will be genetically mapped using crossovers in the chromosomes of the offspring from a primary set of reference pedigrees, which should allow rapid alignment of the corresponding contigs and placement of the loci within the genetic linkage map. Closure of the map will be attained by mapping polymorphisms derived from telomeric YACs and cloned DNAs from the pericentromeric region, and by the use of jumping clones, in situ hybridization and pulsed-field gel mapping. The result will be a map in which cytogenetic assignments, linkage information for genetic loci, and the set of overlapping clones that fully describe chromosome 7 are integrated.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Human Genome Research Institute (NHGRI)
Type
Specialized Center (P50)
Project #
5P50HG000201-07
Application #
5213003
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
7
Fiscal Year
1996
Total Cost
Indirect Cost

  Publications

Taillon-Miller, P; Bauer-Sardina, I; Saccone, N L et al. (2000) Juxtaposed regions of extensive and minimal linkage disequilibrium in human Xq25 and Xq28. Nat Genet 25:324-8
Keen, T J; Inglehearn, C F; Patel, R J et al. (1995) Localization of the aquaporin 1 (AQP1) gene within a YAC contig containing the polymorphic markers D7S632 and D7S526. Genomics 25:599-600
Parrish, J E; Ciccodicola, A; Wehhert, M et al. (1995) A muscle-specific DNase I-like gene in human Xq28. Hum Mol Genet 4:1557-64
Green, E D; Maffei, M; Braden, V V et al. (1995) The human obese (OB) gene: RNA expression pattern and mapping on the physical, cytogenetic, and genetic maps of chromosome 7. Genome Res 5:5-12
Krizman, D B; Hofmann, T A; DeSilva, U et al. (1995) Identification of 3'-terminal exons from yeast artificial chromosomes. PCR Methods Appl 4:322-6
Kusaba, H; Kohno, K; Asakuno, K et al. (1995) Functional expression of yeast artificial chromosome-human multidrug resistance genes in mouse cells. Genome Res 5:245-58
Schiebel, K; Mertz, A; Winkelmann, M et al. (1994) Localization of the adenine nucleotide translocase gene ANT2 to chromosome Xq24-q25 with tight linkage to DXS425. Genomics 24:605-6
Umbricht, C B; Griffin, C A; Hawkins, A L et al. (1994) High-resolution genomic mapping of the three human replication protein A genes (RPA1, RPA2, and RPA3). Genomics 20:249-57
Green, E D; Idol, J R; Mohr-Tidwell, R M et al. (1994) Integration of physical, genetic and cytogenetic maps of human chromosome 7: isolation and analysis of yeast artificial chromosome clones for 117 mapped genetic markers. Hum Mol Genet 3:489-501
Maraia, R J; Sasaki-Tozawa, N; Driscoll, C T et al. (1994) The human Y4 small cytoplasmic RNA gene is controlled by upstream elements and resides on chromosome 7 with all other hY scRNA genes. Nucleic Acids Res 22:3045-52

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