Abstract

We propose to establish a center to build genomic DNA arrays and develop novel technologies that will use these arrays for the large-scale functional analysis of the human genome. 0.3-1.4 kb fragments of nonrepetitive DNA from each of chromosomes 22, 21, 20, 19,7, 17, and perhaps the X chromosome will be prepared by PCR and attached to microscope slides. The arrays will be used to develop technologies for the large-scale mapping of 1) Transcribed sequences. 2) Binding sites of chromosomal proteins 3) Origins of replication. 4) Genetic mutation and variation. A web-accessible database will be constructed to house the information generated in this study; data from other studies will also be integrated into the database. The arrays and technologies will be made available throughout both the Yale University and the larger scientific community. They will be integrated into our training programs for postdoctoral fellows, graduate students and undergraduates at Yale. We expect these procedures to be applicable to the analysis of the entire human genome and the genomes of many other organisms.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Human Genome Research Institute (NHGRI)
Type
Specialized Center (P50)
Project #
1P50HG002357-01
Application #
6362104
Study Section
Ethical, Legal, Social Implications Review Committee (GNOM)
Program Officer
Schloss, Jeffery
Project Start
2001-09-01
Project End
2006-08-31
Budget Start
2001-09-01
Budget End
2002-08-31
Support Year
1
Fiscal Year
2001
Total Cost
$2,517,488
Indirect Cost

  Institution

Name
Yale University
Department
Physiology
Type
Schools of Arts and Sciences
DUNS #
082359691
City
New Haven
State
CT
Country
United States
Zip Code
06520

  Publications

Keating, Brendan J; Pereira, Alexandre C; Snyder, Michael et al. (2018) Applying genomics in heart transplantation. Transpl Int 31:278-290
Carter, Ava C; Chang, Howard Y; Church, George et al. (2017) Challenges and recommendations for epigenomics in precision health. Nat Biotechnol 35:1128-1132
Csabai, Zsolt; Takács, Irma F; Snyder, Michael et al. (2017) Evaluation of the impact of ul54 gene-deletion on the global transcription and DNA replication of pseudorabies virus. Arch Virol 162:2679-2694
Moldován, Norbert; Balázs, Zsolt; Tombácz, Dóra et al. (2017) Multi-platform analysis reveals a complex transcriptome architecture of a circovirus. Virus Res 237:37-46
Esplin, Edward D; Chaib, Hassan; Haney, Michael et al. (2015) 46,XY disorders of sex development and congenital diaphragmatic hernia: a case with dysmorphic facies, truncus arteriosus, bifid thymus, gut malrotation, rhizomelia, and adactyly. Am J Med Genet A 167:1360-4
Menon, Rajasree; Im, Hogune; Zhang, Emma Yue et al. (2014) Distinct splice variants and pathway enrichment in the cell-line models of aggressive human breast cancer subtypes. J Proteome Res 13:212-27
Haraksingh, Rajini R; Jahanbani, Fereshteh; Rodriguez-Paris, Juan et al. (2014) Exome sequencing and genome-wide copy number variant mapping reveal novel associations with sensorineural hereditary hearing loss. BMC Genomics 15:1155
Chen, Rui; Snyder, Michael (2013) Promise of personalized omics to precision medicine. Wiley Interdiscip Rev Syst Biol Med 5:73-82
Hardee, Jennifer; Ouyang, Zhengqing; Zhang, Yuping et al. (2013) STAT3 targets suggest mechanisms of aggressive tumorigenesis in diffuse large B-cell lymphoma. G3 (Bethesda) 3:2173-85
Qian, Feng; Chung, Lisa; Zheng, Wei et al. (2013) Identification of genes critical for resistance to infection by West Nile virus using RNA-Seq analysis. Viruses 5:1664-81

Showing the most recent 10 out of 73 publications