Core - Experimental Laboratories

Brigham, Kenneth L.

Abstract

This Core Area is designed to provide facilities and personnel for work proposed in many projects which require similar experimental and analytical support. The Core will provide large animal experimental and routine analytical laboratories for all experiments and routine analytical laboratories for all experiments in the Program involving either chronic or acute sheep preparations. In addition, analyses of lipid peroxidation products and radioimmunoassay of eicosanoids from animals and from in vitro experiments will be done in this Core as well as routine measurements of granulocyte chemotaxis and superoxide production. The Core also includes facilities and personnel for measurement of antioxidant systems and proteinase/antiproteinase concentrations in material from animal and in vitro experiments. Concentration of activities necessary for a large number of projects in a Core Area enables efficient use of personnel, equipment and space and enhances interactions among investigators.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Heart, Lung, and Blood Institute (NHLBI)
• Type: Specialized Center (P50)
• Project #: 2P50HL019153-16
• Application #: 3843877
• Support Year: 16
• Fiscal Year: 1992

Institution

• Name: Vanderbilt University Medical Center
• DUNS #: 004413456
• City: Nashville
• State: TN
• Country: United States
• Zip Code: 37212

Publications

Brigham, K L; Lane, K B; Meyrick, B et al. (2000) Transfection of nasal mucosa with a normal alpha1-antitrypsin gene in alpha1-antitrypsin-deficient subjects: comparison with protein therapy. Hum Gene Ther 11:1023-32

Conner, B D; Bernard, G R (2000) Acute respiratory distress syndrome. Potential pharmacologic interventions. Clin Chest Med 21:563-87

Mangialardi, R J; Martin, G S; Bernard, G R et al. (2000) Hypoproteinemia predicts acute respiratory distress syndrome development, weight gain, and death in patients with sepsis. Ibuprofen in Sepsis Study Group. Crit Care Med 28:3137-45

Brigham, K L; Stecenko, A A (2000) Gene therapy for acute lung injury. Intensive Care Med 26 Suppl 1:S119-23

Arons, M M; Wheeler, A P; Bernard, G R et al. (1999) Effects of ibuprofen on the physiology and survival of hypothermic sepsis. Ibuprofen in Sepsis Study Group. Crit Care Med 27:699-707

Peters, M T; Brigham, K L; King, G A et al. (1999) Optimization of cationic liposome-mediated gene transfer to human bronchial epithelial cells expressing wild-type or abnormal cystic fibrosis transmembrane conductance regulator (CFTR). Exp Lung Res 25:183-97

Wheeler, A P; Bernard, G R (1999) Treating patients with severe sepsis. N Engl J Med 340:207-14

Snapper, J R; Trochtenberg, D S; Hwang, Y S et al. (1999) Effect of pulmonary edema on tracheal diameter. Respiration 66:522-7

Dupont, W D; Plummer Jr, W D (1998) Power and sample size calculations for studies involving linear regression. Control Clin Trials 19:589-601

Clark, M P; Chow, C W; Rinaldo, J E et al. (1998) Multiple domains for initiator binding proteins TFII-I and YY-1 are present in the initiator and upstream regions of the rat XDH/XO TATA-less promoter. Nucleic Acids Res 26:2813-20