Abstract

The Morphology/Morphometry Core Laboratory provides access to instrumentation, technical assistance, instruction and consultation for SCOR investigators conducting cell biological and morphologic studies at the histologic and ultrastructural level. This laboratory maintains a full range of light and electron microscopes, ancillary apparatus and preparative resources, as well as hardware and software for computer- assisted image, morphometric and densitometric analyses. The laboratory is staffed by well-qualified, experienced technicians who will process tissues for light and transmission electron microscopy, including immunostaining. The Core Director will perform the majority of morphometric analyses needed. All of the proposed projects will rely substantially on the resources available in this unit. This Core's diversity and cost-effectiveness are directly attributable to shared support with other research units using this facility, including a Pediatric Pulmonary SCOR (P.I., Thomas F. Boat), PPG (P.I., Richard Boucher) and the Center for Environmental Medicine and Lung Biology (through a cooperative agreement between U.N.C. and the U.S. Environmental Protection Agency).

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Specialized Center (P50)
Project #
5P50HL042384-09
Application #
5213773
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
9
Fiscal Year
1996
Total Cost
Indirect Cost

  Publications

Coakley, Raymond D; Grubb, Barbara R; Paradiso, Anthony M et al. (2003) Abnormal surface liquid pH regulation by cultured cystic fibrosis bronchial epithelium. Proc Natl Acad Sci U S A 100:16083-8
Tarran, R; Grubb, B R; Gatzy, J T et al. (2001) The relative roles of passive surface forces and active ion transport in the modulation of airway surface liquid volume and composition. J Gen Physiol 118:223-36
Tarran, R; Grubb, B R; Parsons, D et al. (2001) The CF salt controversy: in vivo observations and therapeutic approaches. Mol Cell 8:149-58
Paradiso, A M; Ribeiro, C M; Boucher, R C (2001) Polarized signaling via purinoceptors in normal and cystic fibrosis airway epithelia. J Gen Physiol 117:53-67
Matsui, H; Davis, C W; Tarran, R et al. (2000) Osmotic water permeabilities of cultured, well-differentiated normal and cystic fibrosis airway epithelia. J Clin Invest 105:1419-27
Huang, P; Trotter, K; Boucher, R C et al. (2000) PKA holoenzyme is functionally coupled to CFTR by AKAPs. Am J Physiol Cell Physiol 278:C417-22
Bartlett, J S; Kleinschmidt, J; Boucher, R C et al. (1999) Targeted adeno-associated virus vector transduction of nonpermissive cells mediated by a bispecific F(ab'gamma)2 antibody. Nat Biotechnol 17:181-6
Grubb, B R (1999) Ion transport across the normal and CF neonatal murine intestine. Am J Physiol 277:G167-74
Noone, P G; Bennett, W D; Regnis, J A et al. (1999) Effect of aerosolized uridine-5'-triphosphate on airway clearance with cough in patients with primary ciliary dyskinesia. Am J Respir Crit Care Med 160:144-9
Paradiso, A M; Brown, H A; Ye, H et al. (1999) Heterogeneous responses of cell Ca2+ in human airway epithelium. Exp Lung Res 25:277-90

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