Abstract

The Cell Processing Laboratory will transform and maintain peripheral blood lymphocytes for DNA analysis; culture and maintain frog oocytes for heterologous expression experiments; prepare post-infarct myocyte hypertrophy/heart failure/sudden death models in the rabbit; and isolate cardiac ventricular myocytes suitable for electrophysiological and confocal microscopic study from mice and rabbits. The lymphocyte and oocyte work is a continuation of work ongoing over the previous years. The post infarct/hypertrophy/sudden death model in the rabbit is a recent development, and the model will be further refined by production of larger infarcts into one in which sudden death and heart failure are more common. Newly developed methodology will be applied to obtain single ventricular myocytes from mice and rabbits suitable for studies.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Specialized Center (P50)
Project #
5P50HL052338-07
Application #
6420542
Study Section
Project Start
2001-01-01
Project End
2001-12-31
Budget Start
Budget End
Support Year
7
Fiscal Year
2001
Total Cost
$206,722
Indirect Cost

  Institution

Name
University of Utah
Department
Type
DUNS #
City
Salt Lake City
State
UT
Country
United States
Zip Code
84112

  Publications

Lux, Robert L; Gettes, Leonard S (2011) Repolarization heterogeneity and rate dependency in a canine rapid pacing model of heart failure. J Electrocardiol 44:730-5
Lux, Robert L; Pope 3rd, C Arden (2009) Air pollution effects on ventricular repolarization. Res Rep Health Eff Inst :3-20; discussion 21-8
Segerson, Nathan M; Litwin, Sheldon E; Daccarett, Marcos et al. (2008) Scatter in repolarization timing predicts clinical events in post-myocardial infarction patients. Heart Rhythm 5:208-14
Valencik, Maria L; Zhang, Dongfang; Punske, Bonnie et al. (2006) Integrin activation in the heart: a link between electrical and contractile dysfunction? Circ Res 99:1403-10
Lux, Robert L; Gettes, Leonard S; Mason, Jay W (2006) Understanding proarrhythmic potential in therapeutic drug development: alternate strategies for measuring and tracking repolarization. J Electrocardiol 39:S161-4
Spitzer, Kenneth W; Pollard, Andrew E; Yang, Lin et al. (2006) Cell-to-cell electrical interactions during early and late repolarization. J Cardiovasc Electrophysiol 17 Suppl 1:S8-S14
Splawski, Igor; Yoo, Dana S; Stotz, Stephanie C et al. (2006) CACNA1H mutations in autism spectrum disorders. J Biol Chem 281:22085-91
Shusterman, Vladimir; Goldberg, Anna; London, Barry (2006) Upsurge in T-wave alternans and nonalternating repolarization instability precedes spontaneous initiation of ventricular tachyarrhythmias in humans. Circulation 113:2880-7
Skaluba, Stanislaw J; Bray, Bruce E; Litwin, Sheldon E (2005) Close coupling of systolic and diastolic function: combined assessment provides superior prediction of exercise capacity. J Card Fail 11:516-22
Chen, Tiehua; Inoue, Masashi; Sheets, Michael F (2005) Reduced voltage dependence of inactivation in the SCN5A sodium channel mutation delF1617. Am J Physiol Heart Circ Physiol 288:H2666-76

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