Abstract

A self-contained Molecular biology Core Facility will provide services and technology to Projects 1, 2, 3, 4 and 5 and to the Morphology Core. Dr. Billie Moats-Staats will be the Molecular Biology Core Director and responsible investigator of this facility. The Molecular Biology Core Facility will office a molecular techniques consultation service provided by Drs. Moats-Staats, Stiles, D~Ercole and Hu. Wining Yin and Brian Brighton (Technician IIIs) will perform the service functions of this core which include probe preparation, cloning and mapping techniques, bacterial transformations and growups, RNA isolation and analysis, DNA sequencing, PCR, RT-PCR, screening of transgenic mice, luciferase assays, and inventory and storage of plasmid and bacterial stocks. Technology transfer will be conducted by Weining Yin, Brian Brighton, and Moats-Staats.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Specialized Center (P50)
Project #
5P50HL056395-04
Application #
6202503
Study Section
Project Start
1999-12-01
Project End
2000-11-30
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
4
Fiscal Year
2000
Total Cost
$196,240
Indirect Cost

  Institution

Name
University of North Carolina Chapel Hill
Department
Type
DUNS #
608195277
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599

  Publications

Mellow, Thomas E; Murphy, Paula C; Carson, Johnny L et al. (2004) The effect of respiratory synctial virus on chemokine release by differentiated airway epithelium. Exp Lung Res 30:43-57
Thai, C H; Gambling, T M; Carson, J L (2002) Freeze fracture study of airway epithelium from patients with primary ciliary dyskinesia. Thorax 57:363-5
Veness-Meehan, Kathleen A; Pierce, Richard A; Moats-Staats, Billie M et al. (2002) Retinoic acid attenuates O2-induced inhibition of lung septation. Am J Physiol Lung Cell Mol Physiol 283:L971-80
Carson, Johnny L; Reed, William; Lucier, Thomas et al. (2002) Axonemal dynein expression in human fetal tracheal epithelium. Am J Physiol Lung Cell Mol Physiol 282:L421-30
Kazachkov, Mikhail Y; Hu, P C; Carson, Johnny L et al. (2002) Release of cytokines by human nasal epithelial cells and peripheral blood mononuclear cells infected with Mycoplasma pneumoniae. Exp Biol Med (Maywood) 227:330-5
Noah, Terry L; Ivins, Sally S; Murphy, Paula et al. (2002) Chemokines and inflammation in the nasal passages of infants with respiratory syncytial virus bronchiolitis. Clin Immunol 104:86-95
Price, W A; Lee, E; Maynor, A et al. (2001) Relation between serum insulinlike growth factor-1, insulinlike growth factor binding protein-2, and insulinlike growth factor binding protein-3 and nutritional intake in premature infants with bronchopulmonary dysplasia. J Pediatr Gastroenterol Nutr 32:542-9
Volberg, T; Romer, L; Zamir, E et al. (2001) pp60(c-src) and related tyrosine kinases: a role in the assembly and reorganization of matrix adhesions. J Cell Sci 114:2279-89
Noah, T L; Becker, S (2000) Chemokines in nasal secretions of normal adults experimentally infected with respiratory syncytial virus. Clin Immunol 97:43-9
Veness-Meehan, K A; Bottone Jr, F G; Stiles, A D (2000) Effects of retinoic acid on airspace development and lung collagen in hyperoxia-exposed newborn rats. Pediatr Res 48:434-44

Showing the most recent 10 out of 15 publications