Abstract

The Electrophysiology Core will provide centralized services for in vitro electrophysiological studies, including preparation of the in vitro brain slices, patch-clamp recordings, extracellular recordings, and the associated image and data processing plus analysis.
Five aims i n four projects will utilize this Core facility. Whole-cell patch-clamp recordings will be obtained from in vitro rat brain slices from basal forebrain neurons (Projects 2 & 3), ventrolateral preoptic (VLPO) neurons (Project 3) and from mouse tuberornarnmUlary, locus coeruleus and basal forebrain neurons (Project 5). In addition, extracellular recordings of hippocampal field potentials will be obtained from the CA1 region (Projects 2 & 4). The centralization of this resource makes the technique of in vitro electrophysiological recording available to multiple investigators in an environment that facilitates standardization of recording conditions across all projects.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Specialized Center (P50)
Project #
2P50HL060292-06
Application #
6823467
Study Section
Special Emphasis Panel (ZHL1-CSR-S (M1))
Project Start
2003-09-08
Project End
2008-08-31
Budget Start
2003-09-08
Budget End
2004-08-31
Support Year
6
Fiscal Year
2003
Total Cost
$98,600
Indirect Cost

  Institution

Name
Brigham and Women's Hospital
Department
Type
DUNS #
030811269
City
Boston
State
MA
Country
United States
Zip Code
02115

  Publications

Zielinski, Mark R; Gerashchenko, Dmitry; Karpova, Svetlana A et al. (2017) The NLRP3 inflammasome modulates sleep and NREM sleep delta power induced by spontaneous wakefulness, sleep deprivation and lipopolysaccharide. Brain Behav Immun 62:137-150
Cori, Jennifer M; Thornton, Therese; O'Donoghue, Fergal J et al. (2017) Arousal-Induced Hypocapnia Does Not Reduce Genioglossus Activity in Obstructive Sleep Apnea. Sleep 40:
Chen, Michael C; Ferrari, Loris; Sacchet, Matthew D et al. (2015) Identification of a direct GABAergic pallidocortical pathway in rodents. Eur J Neurosci 41:748-59
Kim, Youngsoo; Elmenhorst, David; Weisshaupt, Angela et al. (2015) Chronic sleep restriction induces long-lasting changes in adenosine and noradrenaline receptor density in the rat brain. J Sleep Res 24:549-558
Zielinski, Mark R; Kim, Youngsoo; Karpova, Svetlana A et al. (2014) Chronic sleep restriction elevates brain interleukin-1 beta and tumor necrosis factor-alpha and attenuates brain-derived neurotrophic factor expression. Neurosci Lett 580:27-31
Lim, Andrew S P; Ellison, Brian A; Wang, Joshua L et al. (2014) Sleep is related to neuron numbers in the ventrolateral preoptic/intermediate nucleus in older adults with and without Alzheimer's disease. Brain 137:2847-61
Kim, Youngsoo; Chen, Lichao; McCarley, Robert W et al. (2013) Sleep allostasis in chronic sleep restriction: the role of the norepinephrine system. Brain Res 1531:9-16
Zielinski, M R; Kim, Y; Karpova, S A et al. (2013) Sleep active cortical neurons expressing neuronal nitric oxide synthase are active after both acute sleep deprivation and chronic sleep restriction. Neuroscience 247:35-42
McCoy, John G; Christie, Michael A; Kim, Youngsoo et al. (2013) Chronic sleep restriction impairs spatial memory in rats. Neuroreport 24:91-5
McKenna, James Timothy; Christie, Michael A; Jeffrey, Brianne A et al. (2012) Chronic ramelteon treatment in a mouse model of Alzheimer's disease. Arch Ital Biol 150:5-14

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