The Mouse Core for this SCOR proposal is built on and integrated with four existing Cores or Services of the Molecular Cardiology Research Institute at New England Medical Center and Tufts University School of Medicine. These four components include: 1) the MCRI Transgenic Mouse Facility 2) The Mouse Tissue Histology Service, 3) the MCRI Mouse Physiology Core and 4) the MCRI Cell Culture Facility. The Mouse Core serves and is closely integrated with Project s 3, 4 and 5 of this SCOR proposal and details regarding experimental interactions may be found in these 3 projects. Descriptions of each of the four MCRI components upon which the SCOR Mouse Core is built follow. MCRI Transgenic Facility (Janis Lem, Ph.D., Director): The MCRI Transgenic facility is housed in a newly constructed 2500 square foot that houses the capabilities to produce both standard transgenic mice by pronuclear microinjection and knockout/knockin mice by blastocyst injection. Contained within the facility is dedicated animal housing, a microinjection lab, a molecular biology lab, and a tissue culture room, along with a cold room, conference room, and office space. The animal room has a capacity of -500 state-of-the-art self-ventilated cages (3,500 mice) to house stud and vasectomized males and donor and recipient female mice used in the production of transgenic and knockout/knockin mice. Dr. Mary Ellenberger, DVM, oversees the care and maintenance of animals, fully in accordance with NIH and institutional guidelines. The investigators Institution is accredited by AAALAC and has current PHS Animal Welfare Assurance.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Specialized Center (P50)
Project #
5P50HL063494-05
Application #
6858702
Study Section
Special Emphasis Panel (ZHL1)
Project Start
2004-02-01
Project End
2005-01-31
Budget Start
2004-02-01
Budget End
2005-01-31
Support Year
5
Fiscal Year
2004
Total Cost
$258,689
Indirect Cost
Name
Tufts University
Department
Type
DUNS #
079532263
City
Boston
State
MA
Country
United States
Zip Code
02111
Demissie, Serkalem; Cupples, L Adrienne; Shearman, Amanda M et al. (2006) Estrogen receptor-alpha variants are associated with lipoprotein size distribution and particle levels in women: the Framingham Heart Study. Atherosclerosis 185:210-8
Shearman, Amanda M; Cooper, Jackie A; Kotwinski, Paul J et al. (2006) Estrogen receptor alpha gene variation is associated with risk of myocardial infarction in more than seven thousand men from five cohorts. Circ Res 98:590-2
Yang, Qiong; Lai, Chao-Qiang; Parnell, Laurence et al. (2005) Genome-wide linkage analyses and candidate gene fine mapping for HDL3 cholesterol: the Framingham Study. J Lipid Res 46:1416-25
Peter, Inga; Shearman, Amanda M; Zucker, Deborah R et al. (2005) Variation in estrogen-related genes and cross-sectional and longitudinal blood pressure in the Framingham Heart Study. J Hypertens 23:2193-200
Shearman, Amanda M; Demissie, Serkalem; Cupples, L Adrienne et al. (2005) Tobacco smoking, estrogen receptor alpha gene variation and small low density lipoprotein level. Hum Mol Genet 14:2405-13
Peter, Inga; Shearman, Amanda M; Vasan, Ramachandran S et al. (2005) Association of estrogen receptor beta gene polymorphisms with left ventricular mass and wall thickness in women. Am J Hypertens 18:1388-95
Jaffe, Iris Z; Mendelsohn, Michael E (2005) Angiotensin II and aldosterone regulate gene transcription via functional mineralocortocoid receptors in human coronary artery smooth muscle cells. Circ Res 96:643-50
Mendelsohn, Michael E; Karas, Richard H (2005) Molecular and cellular basis of cardiovascular gender differences. Science 308:1583-7
Georgescu, Serban P; Li, Joyce H; Lu, Qing et al. (2005) Modulator recognition factor 1, an AT-rich interaction domain family member, is a novel corepressor for estrogen receptor alpha. Mol Endocrinol 19:2491-501
Shearman, Amanda M; Cooper, Jackie A; Kotwinski, Paul J et al. (2005) Estrogen receptor alpha gene variation and the risk of stroke. Stroke 36:2281-2

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