The main objective of this Core is to provide the investigators in this program project group with a complete range of expertise, training, reagents, and equipment to accomplish the imaging of histologic, molecular, and mRNA targets in fixed and living cells and tissues. This Core supports the SCCOR's six research projects with services related to optical detection, visualization, and quantitative microscopy. It offers access to experienced use of the complete resources of the Johns Hopkins University School of Medicine Microscope Facility (which includes an excellent electron microscope facility) and the Department of Medicine's Multiphoton Laser Scanning Microscope Facility. Dr. Tuder has assembled the personnel and laboratory facilities to satisfy a wide range of experimental imaging needs. Core C personnel have professional experience spanning the fields of tissue preparation, cell culture, differential detergent extraction and cytoskeletal preservation, cell component-specific staining, microscopy and digital image analysis. The technical staff of the Core provide essential support to this SCCOR application. The core facility features access to multiple microscopes that utilize both Windows PCs and Macintosh work stations, extensive software for image analysis and visualization, and software and hardware for producing manuscript-ready images and figures in hard copy. Specific competencies provided by this Core include: consultation on experimental design, reagents for immunolabeling and fluorescence protein chimera expression, specimen preparation, biopsy processing, in situ hybridization, autoradiographic studies of radio-labeled material in tissues, immunocytochemical labeling for light, epiflurorescence, multiphoton, or electron microscopic examination, transmitted light and spinning disc confocal implementation of two-and three-dimensional image processing, analysis algorithms and quantitative image analyses, three-dimensional reconstruction and stereoscopic visualization, and graphics services involving digital imaging. This core will prepare and analyze specimens from a broad range of research endeavors. Importantly, during the preparation of preliminary data for this proposal, this Molecular Pathology Core has demonstrated that its flexibility, expertise, and resources are available and essential to all of projects in this SCCOR.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Specialized Center (P50)
Project #
1P50HL073994-01
Application #
6820152
Study Section
Special Emphasis Panel (ZHL1-CSR-R (M1))
Project Start
2003-09-30
Project End
2008-06-30
Budget Start
2003-09-30
Budget End
2004-06-30
Support Year
1
Fiscal Year
2003
Total Cost
$113,517
Indirect Cost
Name
Johns Hopkins University
Department
Type
DUNS #
001910777
City
Baltimore
State
MD
Country
United States
Zip Code
21218
Heins, Sara E; Wozniak, Amy W; Colantuoni, Elizabeth et al. (2018) Factors associated with missed assessments in a 2-year longitudinal study of acute respiratory distress syndrome survivors. BMC Med Res Methodol 18:55
Brodsky, Martin B; De, Ishani; Chilukuri, Kalyan et al. (2018) Coordination of Pharyngeal and Laryngeal Swallowing Events During Single Liquid Swallows After Oral Endotracheal Intubation for Patients with Acute Respiratory Distress Syndrome. Dysphagia 33:768-777
Chan, Kitty S; Mourtzakis, Marina; Aronson Friedman, Lisa et al. (2018) Upper arm anthropometrics versus DXA scan in survivors of acute respiratory distress syndrome. Eur J Clin Nutr 72:613-617
Bienvenu, O Joseph; Friedman, Lisa Aronson; Colantuoni, Elizabeth et al. (2018) Psychiatric symptoms after acute respiratory distress syndrome: a 5-year longitudinal study. Intensive Care Med 44:38-47
Chan, Kitty S; Mourtzakis, Marina; Aronson Friedman, Lisa et al. (2018) Evaluating Muscle Mass in Survivors of Acute Respiratory Distress Syndrome: A 1-Year Multicenter Longitudinal Study. Crit Care Med 46:1238-1246
Brodsky, Martin B; Huang, Minxuan; Shanholtz, Carl et al. (2017) Recovery from Dysphagia Symptoms after Oral Endotracheal Intubation in Acute Respiratory Distress Syndrome Survivors. A 5-Year Longitudinal Study. Ann Am Thorac Soc 14:376-383
Ruhl, A Parker; Huang, Minxuan; Colantuoni, Elizabeth et al. (2017) Healthcare Resource Use and Costs in Long-Term Survivors of Acute Respiratory Distress Syndrome: A 5-Year Longitudinal Cohort Study. Crit Care Med 45:196-204
Dinglas, Victor D; Aronson Friedman, Lisa; Colantuoni, Elizabeth et al. (2017) Muscle Weakness and 5-Year Survival in Acute Respiratory Distress Syndrome Survivors. Crit Care Med 45:446-453
Chan, Kitty S; Aronson Friedman, Lisa; Bienvenu, O Joseph et al. (2016) Distribution-based estimates of minimal important difference for hospital anxiety and depression scale and impact of event scale-revised in survivors of acute respiratory failure. Gen Hosp Psychiatry 42:32-5
Bienvenu, O Joseph; Colantuoni, Elizabeth; Mendez-Tellez, Pedro A et al. (2015) Cooccurrence of and remission from general anxiety, depression, and posttraumatic stress disorder symptoms after acute lung injury: a 2-year longitudinal study. Crit Care Med 43:642-53

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