Abstract

The scientific core consists of two major imaging laboratories to support program project members with state of the art equipment and methodologies.
Aim 1. Provide support to investigators to design and carry out anatomically based analyses utilizing quantitative neuroanatomical techniques including stereology based statistically unbiased approaches.
Aim 2. Assist investigators in imaging blood brain barrier breakdown and MMP uregulation, both in vivo and ex vivo, using extravasation of fluorescent indicators.
Aim 3. Assist investigators in utilizing laser speckle contrast imaging in studies of cerebral blood flow and in the application of multi-spectral in-vivo imaging of intrinsic (e.g hemoglobin concentrations) and extrinsic (e.g. plasma leakiness indicators and MMP activatable fluorescence markers) contrast agents. Advance the methodology to provide more robust measures of blood flow and facilitate investigator driven advanced analyses.
Aim 4. Develop robust high-field MRI measurements of CBF in rodents, and assist investigators in the acquisition and analysis of anatomical and functional MRI data. Taken together, these aims both support the scientific aims of the program project as a whole, while the needs of the projects serve to advance the methodology to facilitate investigator driven analyses.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Specialized Center (P50)
Project #
2P50NS010828-29
Application #
6964287
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
2004-08-15
Project End
2009-04-30
Budget Start
2004-08-15
Budget End
2005-04-30
Support Year
29
Fiscal Year
2004
Total Cost
$238,002
Indirect Cost

  Institution

Name
Massachusetts General Hospital
Department
Type
DUNS #
073130411
City
Boston
State
MA
Country
United States
Zip Code
02199

  Publications

Yaseen, Mohammad A; Srinivasan, Vivek J; Gorczynska, Iwona et al. (2015) Multimodal optical imaging system for in vivo investigation of cerebral oxygen delivery and energy metabolism. Biomed Opt Express 6:4994-5007
Miao, Yanying; Liao, James K (2014) Potential serum biomarkers in the pathophysiological processes of stroke. Expert Rev Neurother 14:173-85
Sawada, Naoki; Liao, James K (2014) Rho/Rho-associated coiled-coil forming kinase pathway as therapeutic targets for statins in atherosclerosis. Antioxid Redox Signal 20:1251-67
Hayakawa, Kazuhide; Miyamoto, Nobukazu; Seo, Ji Hae et al. (2013) High-mobility group box 1 from reactive astrocytes enhances the accumulation of endothelial progenitor cells in damaged white matter. J Neurochem 125:273-80
Tanaka, Shin-ichi; Fukumoto, Yoshihiro; Nochioka, Kotaro et al. (2013) Statins exert the pleiotropic effects through small GTP-binding protein dissociation stimulator upregulation with a resultant Rac1 degradation. Arterioscler Thromb Vasc Biol 33:1591-600
Montalvo, J; Spencer, C; Hackathorn, A et al. (2013) ROCK1 & 2 perform overlapping and unique roles in angiogenesis and angiosarcoma tumor progression. Curr Mol Med 13:205-19
Yaseen, Mohammad A; Sakadži?, Sava; Wu, Weicheng et al. (2013) In vivo imaging of cerebral energy metabolism with two-photon fluorescence lifetime microscopy of NADH. Biomed Opt Express 4:307-21
Zhou, Qian; Mei, Yu; Shoji, Takuhito et al. (2012) Rho-associated coiled-coil-containing kinase 2 deficiency in bone marrow-derived cells leads to increased cholesterol efflux and decreased atherosclerosis. Circulation 126:2236-47
Sakadži?, Sava; Roussakis, Emmanuel; Yaseen, Mohammad A et al. (2011) Cerebral blood oxygenation measurement based on oxygen-dependent quenching of phosphorescence. J Vis Exp :
Yaseen, Mohammad A; Srinivasan, Vivek J; Sakadzic, Sava et al. (2011) Microvascular oxygen tension and flow measurements in rodent cerebral cortex during baseline conditions and functional activation. J Cereb Blood Flow Metab 31:1051-63

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