The purpose of this Core is to make available various transgenic and knockout mutants for Projects 1, 2,and 3 of the program. One particular strength of this Transgenic Animal Core is that Dr. Chan (Core Leader)will provide his expertise to the other projects in the use of superoxide dismutases (SOD1, SOD2), intransgenic and knockout mutant mice, and NAD(P)H oxidase mice (gp91phox and p47phox knockout), as wellas oxygen radical-related biochemistry and molecular biology. In addition, Dr. Chan will provide his expertisein primary cell culture of neurons (Project 2), astrocytes (Project 3), and endothelial cells (Project 1) fromrodents (transgenic, knockout, and genetically-matched wild-types). This Core will serve as a centralizedfacility for set-up, for characterization, and for quality control of these primary cell cultures from wild-typeanimals, as well as SOD1 and SOD2 transgenic and knockout mutant mice. In addition, HSP70 transgenicand PARP-1 knockout mice will be bred and genotyped for Project 3 and Project 2, respectively This Corewill also provide expertise in the molecular analysis of gene expression (PCR, RT-PCR, real-time RT-PCR),gel electrophoresis, and enzymatic activity of CuZnSOD, MnSOD, NAD(P)H oxidase, and biochemicalassays in mice and in primary cell cultures. Finally, this Core will generate and characterize the doubletransgenic/knockout mice (SOD1+/- / gp91phox-/-, SOD2+/- / gp91phox-/-, HSP70+/+ / SOD2-/+) for Projects 1and 3. Thus, the Transgenic Animal Core provides a unique vehicle for the projects to achieve the researchgoals employing various transgenic and knockout animals.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Specialized Center (P50)
Project #
2P50NS014543-29
Application #
7382861
Study Section
Special Emphasis Panel (ZNS1-SRB-M (45))
Project Start
2007-07-01
Project End
2012-06-30
Budget Start
2007-08-15
Budget End
2008-07-31
Support Year
29
Fiscal Year
2007
Total Cost
$264,994
Indirect Cost
Name
Stanford University
Department
Type
DUNS #
009214214
City
Stanford
State
CA
Country
United States
Zip Code
94305
Kim, Jong Youl; Kim, Nuri; Yenari, Midori A et al. (2013) Hypothermia and pharmacological regimens that prevent overexpression and overactivity of the extracellular calcium-sensing receptor protect neurons against traumatic brain injury. J Neurotrauma 30:1170-6
Voloboueva, Ludmila A; Emery, John F; Sun, Xiaoyun et al. (2013) Inflammatory response of microglial BV-2 cells includes a glycolytic shift and is modulated by mitochondrial glucose-regulated protein 75/mortalin. FEBS Lett 587:756-62
Sakata, Hiroyuki; Niizuma, Kuniyasu; Wakai, Takuma et al. (2012) Neural stem cells genetically modified to overexpress cu/zn-superoxide dismutase enhance amelioration of ischemic stroke in mice. Stroke 43:2423-9
Tang, Xian Nan; Cairns, Belinda; Kim, Jong Youl et al. (2012) NADPH oxidase in stroke and cerebrovascular disease. Neurol Res 34:338-45
Cairns, Belinda; Kim, Jong Youl; Tang, Xian Nan et al. (2012) NOX inhibitors as a therapeutic strategy for stroke and neurodegenerative disease. Curr Drug Targets 13:199-206
Voloboueva, Ludmila A; Giffard, Rona G (2011) Inflammation, mitochondria, and the inhibition of adult neurogenesis. J Neurosci Res 89:1989-96
Tang, Xian N; Zheng, Zhen; Giffard, Rona G et al. (2011) Significance of marrow-derived nicotinamide adenine dinucleotide phosphate oxidase in experimental ischemic stroke. Ann Neurol 70:606-15
Chen, Hai; Kim, Gab Seok; Okami, Nobuya et al. (2011) NADPH oxidase is involved in post-ischemic brain inflammation. Neurobiol Dis 42:341-8
Yoshioka, Hideyuki; Niizuma, Kuniyasu; Katsu, Masataka et al. (2011) NADPH oxidase mediates striatal neuronal injury after transient global cerebral ischemia. J Cereb Blood Flow Metab 31:868-80
Xiong, Xiaoxing; Barreto, George E; Xu, Lijun et al. (2011) Increased brain injury and worsened neurological outcome in interleukin-4 knockout mice after transient focal cerebral ischemia. Stroke 42:2026-32

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