Progesterone receptors (PR) are expressed by granulosa cells (CG) in the maturing follicle after, but not before, the ovulatory gonadotropin surge in natural [i.e., midcycle surge of luteinizing hormone (LH) and follicle stimulating hormone (FSH)] or artificial [i.e., a midcycle bolus of human chorionic gonadotropin (hCG)] menstrual cycles. This project was designed to determine whether gonadotropins act directly or indirectly via prostaglandins (PG) or progesterone (P) to induce PR expression in nonluteinized CG's. Granulosa cells isolated from gonadotropin-treated rhesus monkeys not receiving an ovulatory stimulus were cultured (8 x 104 cells/well) on fibronectin coated 8-well glass chamber slides in chemically-defined medium containing human low-density lipoprotein (25 fg hLDL/ml) (control) with or without LH (10 or 400 ng/ml), hCG or FSH (400 ng/ml), PGE2, PGF2` or PGA2 (14 fM), trilostane (TRL), a 3 -hydroxy-steroid dehydrogenase inhibitor (250 ng/ml) or flurbiprofin (FLUR), a prostaglandin synthase inhibitor (100 fM). Spent medium was collected daily for 2 days and saved for progesterone determination by RIA. On days 0 and 2, cells were frozen in liquid propane and PR determined by immunocytochemistry. Progesterone production and PR expression was significantly increased (P<0.05) over control values in cultures containing 10 or 400 ng/ml LH and 400 ng/ml FSH or hCG. Trilostane significantly (P<0.05) inhibited P production and tended to decrease the percent of PR-positive cells. Exposure to LH (400 ng/ml) overcame both P production and PR expression inhibition by TRL. PGE2 significantly increased (P<0.05) P production, but none of the PG altered PR expression compared to control values. FLUR had no effect on P production but significantly (P<0.05) decreased the percent PR-positive cells compared to controls. PGE2, as well as LH, prevented the decrease caused by FLUR. Thus, exogenous gonadotropins, but not PG, stimulated PR expression in nonluteinized monkey granulosa cells during culture. However, locally produced PG's may also facilitate PR expression.

National Institute of Health (NIH)
National Center for Research Resources (NCRR)
Primate Research Center Grants (P51)
Project #
Application #
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Toro, C A; Aylwin, C F; Lomniczi, A (2018) Hypothalamic epigenetics driving female puberty. J Neuroendocrinol 30:e12589
Bulgarelli, Daiane L; Ting, Alison Y; Gordon, Brenda J et al. (2018) Development of macaque secondary follicles exposed to neutral red prior to 3-dimensional culture. J Assist Reprod Genet 35:71-79
Prola-Netto, Joao; Woods, Mark; Roberts, Victoria H J et al. (2018) Gadolinium Chelate Safety in Pregnancy: Barely Detectable Gadolinium Levels in the Juvenile Nonhuman Primate after in Utero Exposure. Radiology 286:122-128
Moccetti, Federico; Brown, Eran; Xie, Aris et al. (2018) Myocardial Infarction Produces Sustained Proinflammatory Endothelial Activation in Remote Arteries. J Am Coll Cardiol 72:1015-1026
Blue, Steven W; Winchell, Andrea J; Kaucher, Amy V et al. (2018) Simultaneous quantitation of multiple contraceptive hormones in human serum by LC-MS/MS. Contraception 97:363-369
Jeon, Sookyoung; Li, Qiyao; Rubakhin, Stanislav S et al. (2018) 13C-lutein is differentially distributed in tissues of an adult female rhesus macaque following a single oral administration: a pilot study. Nutr Res :
Slayden, Ov Daniel; Friason, Francis Kathryn E; Bond, Kise Rosen et al. (2018) Hormonal regulation of oviductal glycoprotein 1 (OVGP1; MUC9) in the rhesus macaque cervix. J Med Primatol 47:362-370
Okoye, Afam A; Hansen, Scott G; Vaidya, Mukta et al. (2018) Early antiretroviral therapy limits SIV reservoir establishment to delay or prevent post-treatment viral rebound. Nat Med 24:1430-1440
Jensen, Jeffrey T; Hanna, Carol; Mishler, Emily et al. (2018) Effect of menstrual cycle phase and hormonal treatments on evaluation of tubal patency in baboons. J Med Primatol 47:40-45
Dissen, G A; Adachi, K; Lomniczi, A et al. (2017) Engineering a gene silencing viral construct that targets the cat hypothalamus to induce permanent sterility: An update. Reprod Domest Anim 52 Suppl 2:354-358

Showing the most recent 10 out of 492 publications