In primate brains, only mammalian GnRH (mGnRH) has been detected. Here we report the presence of a second form of GnRH, chicken GnRH-II (cGnRH-II), in the monkey brain. First, four brains from rhesus monkey fetuses at E72-E77 were powdered, combined and extracted with acidic acetone. HPLC and RIA analysis showed the presence of mGnRH (antisera GF-4 and B-6) in fractions 20/21. This elution position matched that of the synthetic peptide. Second, 3 adult brains from stumptail monkeys at ages 14-19 years were combined. Both mGnRH cGnRH-II were detected; cGnRH-II (antiserum 7CR10) was in fraction 26. Third, the diencephalon-medial telencephalon part had the highest content of mGnRH; the midbrain-brain stem and diencephalon-medial telencephalon had equal amounts of cGnRH-II; the cortical regions including the temporal lobe had the lowest content of both peptides. An additional peak of GnRH of unknown structure was detected by antiserum B-6. Fourth, immunocytochemistry with antiserum 675 (J. King and R. Millar) showed the presence of cGnRH-II positive cells in the periaqueductal regions of the midbrain in a rhesus monkey fetus at E85 and in regions surrounding the mesencephalic and rhombencephalic vesicles in a rhesus fetus at E38. Fifth, injection of cGnRH-II into adult rhesus monkeys N-5) resulted in a significant increase the circulating plasma LH concentration at 10 min after injection during the luteal phase, but no change after injection in the follicular phase. We conclude the cGnRH-II as well as mGnRH are present in the monkey brain and that cGnRH-II is capable of releasing LH during the luteal phase. (Findings from this study were presented in 1996 at the International Congress of Endocrinology, held in San Francisco, CA).

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Primate Research Center Grants (P51)
Project #
5P51RR000163-38
Application #
6247189
Study Section
Project Start
1997-05-01
Project End
1998-04-30
Budget Start
1996-10-01
Budget End
1997-09-30
Support Year
38
Fiscal Year
1997
Total Cost
Indirect Cost
Name
Oregon Regional Primate Research Center
Department
Type
DUNS #
City
Beaverton
State
OR
Country
United States
Zip Code
97006
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