This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.Cryopreservation of ovarian tissue with subsequent grafting may circumvent chemotherapy-induced sterility in women. Investigations in a nonhuman primate model will facilitate clinical application. Autografts of ovarian cortical tissue in a rhesus monkey were previously placed at various sites subcutaneously or on the kidney. Follicular development was monitored endocrinologically and oocytes retrieved when possible. Also, an intermediate animal host, as in mouse xenografts, may alleviate the risk of cancer transmission and additionally provide a model to study apoptosis and angiogenesis during the early stages of graft development. Monkey ovarian tissue was transplanted to kidneys of immunodeficient mice. Our present focus is the pretreatment of tissue with the anti-apoptotic agent, S1P, with regards to DNA degradation as measured by TUNEL analysis and apoptosis measured by activated caspase activity. Initial studies examined the effects of S1P in vitro with these apoptotic indices.
Showing the most recent 10 out of 492 publications
