We are developing an animal model of simian T-cell lymphotropic virus (STLV-I) induced disease. We originally identified an African green monkey with lymphoproliferative disease and advanced immunodeficiency disease. This monkey was naturally infected with STLV-IAGM and SIVAGM. It developed hyperplastic lymph nodes that arose from the expansion of STLV-I infected and monoclonally integrated cells, immunosuppression and widespread dissemination of a naturally occurring simian immunodeficiency virus (SIVAGM). Serological screening of our rhesus breeding colony has demonstrated an approximate incidence of 15-20% positive for antibodies to STLV-I. The African green monkey colony was similarly screened, but showed a much higher 51% incidence of STLV-I antibodies. The positives were at least at levels 4-5 fold higher than background. We have isolated virus-producing transformed cell lines from a number of these animals and have utilized them for inoculations into naive animals. The animals presently on this study are recipients of STLV-IAGM, STLV-IRhesus, or STLV-ISooty Mangabey. All animals seroconverted, became PCR and culture positive. There was an apparent lymphocytosis, and lymphocyte subset changes in certain of the animals. However, the virus has now become latent, as would be expected in the natural course of the disease. We have been utilizing various treatments (hormone, and immunosuppresion) to stimulate and reactivate the virus. Results to date suggest some success with this approach. We are monitoring longevity of activation,

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Primate Research Center Grants (P51)
Project #
2P51RR000164-37
Application #
6277409
Study Section
Project Start
1998-09-30
Project End
1999-04-30
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
37
Fiscal Year
1998
Total Cost
Indirect Cost
Name
Tulane University
Department
Type
DUNS #
City
New Orleans
State
LA
Country
United States
Zip Code
70118
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Datta, Dibyadyuti; Bansal, Geetha P; Grasperge, Brooke et al. (2017) Comparative functional potency of DNA vaccines encoding Plasmodium falciparum transmission blocking target antigens Pfs48/45 and Pfs25 administered alone or in combination by in vivo electroporation in rhesus macaques. Vaccine 35:7049-7056
Yi, Fei; Guo, Jia; Dabbagh, Deemah et al. (2017) Discovery of Novel Small-Molecule Inhibitors of LIM Domain Kinase for Inhibiting HIV-1. J Virol 91:
Jorgensen, Matthew J; Lambert, Kelsey R; Breaux, Sarah D et al. (2017) Pair housing of Vervets/African Green Monkeys for biomedical research. Am J Primatol 79:1-10

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