This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.Macrophages and microglia are the major cell types infected in the central nervous system of humans infected with HIV and macaques infected with SIV. Microglia are the resident macrophages of the brain and have been shown to be quite sensitive to even minor disturbances of central nervous system (CNS) homeostasis, and are readily activated. Activation of microglia induces changes in cellular morphology and in the expression of cell surface receptors. CD163 is a member of the scavenger receptor family with cysteine-rich domains (SCRC) identified as a receptor of haptoglobin-hemoglobin (Hp-Hb) complex and exclusively expressed by cells of monocyte-macrophage lineage. We examined the expression of CD163 in vitro and in vivo by multiple techniques and at varying times after SIV infection in animals with or without SIVE. Our data show that CD163 is expressed by cells of monocyte/macrophage lineage including perivascular macrophages but not parenchymal microglia in normal and acutely SIV-infected animals or animals with terminal AIDS without encephalitis. CD163 expression was detected in activated microglia (HLA-DR+) surrounding SIVE lesions in chronically infected macaques with severe encephalitis in the presence of haptoglobin-haemoglobin complex (Hp-Hb) in the tissue suggesting breakdown of the blood-brain-barrier. CD163 expression was also induced in microglia in vitro by stimulation with Hp-Hb complex indicating that the interaction of the Hp-Hb complex is required to trigger the upregulation of CD163. To confirm that activation of microglia was associated with the presence and upregulation of CD163 RNA we treated microglia isolated from rhesus macaque brain with Hp-Hb for 0, 6, 12, 18 and 48 h and performed quantitative real-time PCR. We observed a 2.5 fold increase of CD163 RNA in the microglia treated with haptoglobin-hemoglobin within 18hrs of exposure. We conclude that CD163 is a selective marker of perivascular macrophages in normal macaques and during the early phases of SIV infection. However, latter in infection CD163 also labels microglia that have been activated probably as a result of vascular compromise.

National Institute of Health (NIH)
National Center for Research Resources (NCRR)
Primate Research Center Grants (P51)
Project #
Application #
Study Section
Special Emphasis Panel (ZRR1-CM-8 (01))
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Tulane University
Other Domestic Higher Education
New Orleans
United States
Zip Code
Mahalingam, Ravi; Kaufer, Benedikt B; Ouwendijk, Werner J D et al. (2018) Attenuation of Simian Varicella Virus Infection by Enhanced Green Fluorescent Protein in Rhesus Macaques. J Virol 92:
Kumar, Vinay; Mansfield, Joshua; Fan, Rong et al. (2018) miR-130a and miR-212 Disrupt the Intestinal Epithelial Barrier through Modulation of PPAR? and Occludin Expression in Chronic Simian Immunodeficiency Virus-Infected Rhesus Macaques. J Immunol 200:2677-2689
Parthasarathy, Geetha; Philipp, Mario T (2018) Intracellular TLR7 is activated in human oligodendrocytes in response to Borrelia burgdorferi exposure. Neurosci Lett 671:38-42
McNamara, Ryan P; Costantini, Lindsey M; Myers, T Alix et al. (2018) Nef Secretion into Extracellular Vesicles or Exosomes Is Conserved across Human and Simian Immunodeficiency Viruses. MBio 9:
Calenda, Giulia; Villegas, Guillermo; Barnable, Patrick et al. (2017) MZC Gel Inhibits SHIV-RT and HSV-2 in Macaque Vaginal Mucosa and SHIV-RT in Rectal Mucosa. J Acquir Immune Defic Syndr 74:e67-e74
Datta, Dibyadyuti; Bansal, Geetha P; Grasperge, Brooke et al. (2017) Comparative functional potency of DNA vaccines encoding Plasmodium falciparum transmission blocking target antigens Pfs48/45 and Pfs25 administered alone or in combination by in vivo electroporation in rhesus macaques. Vaccine 35:7049-7056
Yi, Fei; Guo, Jia; Dabbagh, Deemah et al. (2017) Discovery of Novel Small-Molecule Inhibitors of LIM Domain Kinase for Inhibiting HIV-1. J Virol 91:
Jorgensen, Matthew J; Lambert, Kelsey R; Breaux, Sarah D et al. (2017) Pair housing of Vervets/African Green Monkeys for biomedical research. Am J Primatol 79:1-10
Ramesh, Geeta; Martinez, Alejandra N; Martin, Dale S et al. (2017) Effects of dexamethasone and meloxicam on Borrelia burgdorferi-induced inflammation in glial and neuronal cells of the central nervous system. J Neuroinflammation 14:28
Parthasarathy, Geetha; Philipp, Mario T (2017) Receptor tyrosine kinases play a significant role in human oligodendrocyte inflammation and cell death associated with the Lyme disease bacterium Borrelia burgdorferi. J Neuroinflammation 14:110

Showing the most recent 10 out of 352 publications