This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.Objective: To analyze the primary infection dynamics of natural SIV infection among African non-human primates (NHPs) and to identify the major differences with pathogenic SIV infection in macaques.Methods: Five species of African (N=43) and Asian (N=43) NHPs were inoculated with SIV. Mathematical modeling using frequently sampled plasma RNA viral loads (VLs) and peripheral CD4+ lymphocyte counts allowed estimation of infection parameter values.Results: The average levels of peak viremia (7.5 0.7 log copies RNA/mL at 9 1 days) and viral steady state levels were similar in the African and Asian NHPs (5.4 1.1 log copies RNA/mL). The exponential growth stage had viral doubling times (average 10 3 days) similar in all species. The CD4+ lymphocytes declined to similar extent in all species during acute SIV infection. However, the decline in VL was faster in African monkeys indicating that the infected cell loss rate following the peak was faster in nonpathogenic than pathogenic infections (P0.001), indicating that the infected cell half-lifes were shorter in African NHPs (0.8 0.3 days) than in Asian NHPs (2.7 1.6 days). The number of secondary infections was much lower in African NHPs leading to a lower percentage of infected cells in these animals compared with their Asian counterparts.Conclusions: The analysis shows that the viral setpoint is reached more rapidly in African than in Asian NHPs. Our modeling suggests that this is due to a more rapid loss of productively infected cells in Africans NHPs as compared to Asian NHPs.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Primate Research Center Grants (P51)
Project #
2P51RR000164-47
Application #
7716301
Study Section
Special Emphasis Panel (ZRR1-CM-8 (01))
Project Start
2008-07-21
Project End
2009-04-30
Budget Start
2008-07-21
Budget End
2009-04-30
Support Year
47
Fiscal Year
2008
Total Cost
$64,630
Indirect Cost
Name
Tulane University
Department
Type
Other Domestic Higher Education
DUNS #
053785812
City
New Orleans
State
LA
Country
United States
Zip Code
70118
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