This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.The major aims of this project are to use electron microscopic techniques to determine the localization of the D1 and D5 dopamine receptors and their signaling proteins within primate prefrontal cortex, and to identify any physical interactions between these receptors and their downstream effector proteins utilizing immunoprecipitation methods. The prefrontal cortex has a complicated circuitry, and understanding where these two receptors are located within specific elements of this circuitry will deepen our understanding of how D1 family receptor signaling effects prefrontal functioning. In the past year I have determined the localization of D1 and D5 in prefrontal cortex (PFC) neuropil of layers I, III and V of the primate. Additionally, I have determined their co-localization within dendritic spines and axon terminals of layer III. Moreover, I have nearly completed determining D1 and D5 localization to parvalbumin and calretinin interneuron dendrites and axon terminals. Significant progress has been made in determining the localization of protein phosphatase-1 isoforms to parvalbumin and calretinin interneurons.
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