HIV-2287 is a viral isolate derived from in vivo passage 2 of HIV-2EHO in Macaca nemestrina. Part of this experiment was to evaluate the pathogenic potential of this virus in other macaque species. Two M. mulatta and two M. fascicularis were inoculated with 5,000 TCID intravenously. The two M. mulatta became infected at very low levels and did not show any depletion of the CD4+ cell population. However, the two M. fascicularis became infected at higher levels in the peripheral blood, and both showed dramatic loss of the CD4+ cell population, one becoming virtually depleted within 4 weeks post-inoculation. This species variation warrants more investigation. Another part of this project is evaluation of the infectivity and pathogenesis of other lentivirus isolates. A lymph node had been isolated from another macaque that was part of the in vivo passage of HIV-2EHO (passage 3). A viral isolate recovered from this lymph node was inoculated into one M. nemestrina as part of this project. This isolate produced infection and CD4+ cell depletion in a time frame comparable to the HIV-2287. This may expand the available pathogenic isolates for future research.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Primate Research Center Grants (P51)
Project #
5P51RR000166-38
Application #
6116341
Study Section
Project Start
1999-05-01
Project End
2000-04-30
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
38
Fiscal Year
1999
Total Cost
Indirect Cost
Name
University of Washington
Department
Type
DUNS #
135646524
City
Seattle
State
WA
Country
United States
Zip Code
98195
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