AIDS-related studies in macaque models for antiretroviral or vaccine studies require well-characterized virus stocks. Because our original virus stock has remained limited, we urgently need a new virus stock for proposed or ongoing research projects. Thus, we have propagated a large quantity of uncloned SIVmne in primary PBMCs from na?ve macaque donors. Before using the stock, we must determine its animal infectious dose (AID). In this project, the new SIVmne stock was titered and inoculated intravenously and intrarectally into Macaca fascicularis. The AID for intravenous inoculation is being studied in five juvenile macaques, one with a dose of 103 TCID and two each with doses of 102 and 10 TCID. The AID for intrarectal inoculation is being studied in three male and three female juvenile macaques, one male and one female each at doses of 104, 103, and 102 TCID. The minimum infectious doses and AIDS-like disease in inoculated macaques will be determined in the follo w-up study in the coming months. FUNDING NIH contract AI65311 and NIH grant RR00166.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Primate Research Center Grants (P51)
Project #
3P51RR000166-38S1
Application #
6219664
Study Section
Project Start
1999-05-01
Project End
2000-04-30
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
38
Fiscal Year
1999
Total Cost
Indirect Cost
Name
University of Washington
Department
Type
DUNS #
135646524
City
Seattle
State
WA
Country
United States
Zip Code
98195
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