Abstract

To determine the effect of agents which perturb the cytoskeleton of the trabecular meshwork so as to increase aqueous outflow on contraction of the ciliary muscle in vitro RESULTS H-7 and inhibits actin-based contraction in monkey ciliary muscle DISCUSSION Ciliary muscle strips isolated from the globes of rhesus monkeys which were being euthanized were mounted in a contraction chamber. After equilibration, 105M pilocapine hydrochloride, a just maximal dose for inducing contraction, was perfused throughout the chamber for 15 min. Successively higher concentrations of H-7, an agent which disrupts actomyosin contractility, were then added to the pilocapine bath. The change in force from resting tension was compared to that with pilocarpine alone. FUTURE DIRECTIONS Further characterization of the mechanism of action of H-7 and other cytoskeletally active compounds on anterior ocular segment functions will facilitate their development as antiglaucoma agents. KEY WORDS glaucoma therapy, pilocarpine, actomyosin FUNDING NEI EY02698 PUBLICATIONS Tian B, Millar C, Kaufman PL, Bershadsky A, Becker E, Geiger B H-7 effects on the iris and ciliary muscle in monkeys. Arch Ophthalmol. 116 1070-1077, 1998. [J]

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Primate Research Center Grants (P51)
Project #
5P51RR000167-40
Application #
6312917
Study Section
Project Start
1976-06-01
Project End
2002-04-30
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
40
Fiscal Year
2000
Total Cost
$82,200
Indirect Cost

  Institution

Name
University of Wisconsin Madison
Department
Type
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715

  Publications

Ellis-Connell, Amy L; Balgeman, Alexis J; Zarbock, Katie R et al. (2018) ALT-803 Transiently Reduces Simian Immunodeficiency Virus Replication in the Absence of Antiretroviral Treatment. J Virol 92:
Park, Mi Ae; Jung, Ho Sun; Slukvin, Igor (2018) Genetic Engineering of Human Pluripotent Stem Cells Using PiggyBac Transposon System. Curr Protoc Stem Cell Biol 47:e63
Kang, HyunJun; Mesquitta, Walatta-Tseyon; Jung, Ho Sun et al. (2018) GATA2 Is Dispensable for Specification of Hemogenic Endothelium but Promotes Endothelial-to-Hematopoietic Transition. Stem Cell Reports 11:197-211
Rhoads, Timothy W; Burhans, Maggie S; Chen, Vincent B et al. (2018) Caloric Restriction Engages Hepatic RNA Processing Mechanisms in Rhesus Monkeys. Cell Metab 27:677-688.e5
Ellis, Amy; Balgeman, Alexis; Rodgers, Mark et al. (2017) Characterization of T Cells Specific for CFP-10 and ESAT-6 in Mycobacterium tuberculosis-Infected Mauritian Cynomolgus Macaques. Infect Immun 85:
Rodrigues, Michelle A (2017) Female Spider Monkeys (Ateles geoffroyi) Cope with Anthropogenic Disturbance Through Fission-Fusion Dynamics. Int J Primatol 38:838-855
Buechler, Connor R; Bailey, Adam L; Lauck, Michael et al. (2017) Genome Sequence of a Novel Kunsagivirus (Picornaviridae: Kunsagivirus) from a Wild Baboon (Papio cynocephalus). Genome Announc 5:
Wu, Hong; Whritenour, Jessica; Sanford, Jonathan C et al. (2017) Identification of MHC Haplotypes Associated with Drug-induced Hypersensitivity Reactions in Cynomolgus Monkeys. Toxicol Pathol 45:127-133
Shackman, A J; Fox, A S; Oler, J A et al. (2017) Heightened extended amygdala metabolism following threat characterizes the early phenotypic risk to develop anxiety-related psychopathology. Mol Psychiatry 22:724-732
Kalin, Ned H (2017) Mechanisms underlying the early risk to develop anxiety and depression: A translational approach. Eur Neuropsychopharmacol 27:543-553

Showing the most recent 10 out of 528 publications