This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. To compare two of the more commonly used cell lines, BeWo and JEG3, by microarray analysis in order to determine their number of differentially expressed genes. Trophoblast cell lines are important research tools used for the study of placental cell function. Because the methods used to create the various cell lines are diverse, it would be helpful to understand if each cell line should be regarded as completely unique, or if some cell lines may be used interchangeably. Results indicated that approximately 2,700 genes were differentially expressed between the cell lines, with principle differences observed in the biological processes of response to stress, cell adhesion, and protein and nucleobase metabolism. These data suggest that BeWo and JEG3 cell lines, and perhaps other trophoblast cell lines, may be sufficiently dissimilar from each other such that different experimental results could arise depending upon which cell line was selected. This research used WNPRC Animal Services and Research Services.
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