Abstract

Significance Objective The objective of this preliminary study is to develop a primate model to study human respiratory syncytial virus (RSV) infection. The model will be used to study pathogenesis of the disease and to test new vaccine strategies. To develop this model we used a mouse-adapted human RSV isolate and infected an infant rhesus monkey by aerosol. The monkey was monitored daily for development of clinical signs and virus shedding was evaluated daily by culturing swabs taken from the nasal cavity. Results The monkey failed to become infected. Currently we are developing an assay (ELISA) to measure RSV-specific antibody titers in monkeys, so that we can test a large number of monkeys at the Primate Center to see if the population has been exposed to RSV from human contact, and therefore might have some resistance. Future Directions In a future experiment we will use a recent human RSV isolate to expose a monkey and will re-isolate the virus for use in future experiments if the infection attempt is successful. Future plans include development of a primate model of allergic asthma, which will be used along with the RSV-infection model to study the interrelationship between RSV infection and allergic asthma. KEYWORDS respiratory syncytial virus, allergy, asthma, ELISA

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Primate Research Center Grants (P51)
Project #
5P51RR000169-37
Application #
6277956
Study Section
Project Start
1998-05-01
Project End
1999-04-30
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
37
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
University of California Davis
Department
Type
DUNS #
094878337
City
Davis
State
CA
Country
United States
Zip Code
95618

  Publications

Comrie, Alison E; Gray, Daniel T; Smith, Anne C et al. (2018) Different macaque models of cognitive aging exhibit task-dependent behavioral disparities. Behav Brain Res 344:110-119
Day, George Q; Ng, Jillian; Oldt, Robert F et al. (2018) DNA-based Determination of Ancestry in Cynomolgus Macaques (Macaca fascicularis). J Am Assoc Lab Anim Sci 57:432-442
Carroll, Timothy D; Jegaskanda, Sinthujan; Matzinger, Shannon R et al. (2018) A Lipid/DNA Adjuvant-Inactivated Influenza Virus Vaccine Protects Rhesus Macaques From Uncontrolled Virus Replication After Heterosubtypic Influenza A Virus Challenge. J Infect Dis 218:856-867
Midic, Uros; VandeVoort, Catherine A; Latham, Keith E (2018) Determination of single embryo sex in Macaca mulatta and Mus musculus RNA-Seq transcriptome profiles. Physiol Genomics 50:628-635
Almodovar, Sharilyn; Swanson, Jessica; Giavedoni, Luis D et al. (2018) Lung Vascular Remodeling, Cardiac Hypertrophy, and Inflammatory Cytokines in SHIVnef-Infected Macaques. Viral Immunol 31:206-222
Ciupe, Stanca M; Miller, Christopher J; Forde, Jonathan E (2018) A Bistable Switch in Virus Dynamics Can Explain the Differences in Disease Outcome Following SIV Infections in Rhesus Macaques. Front Microbiol 9:1216
Kyle, Colin T; Stokes, Jared; Bennett, Jeffrey et al. (2017) Cytoarchitectonically-driven MRI atlas of nonhuman primate hippocampus: Preservation of subfield volumes in aging. Hippocampus :
Moadab, Gilda; Bliss-Moreau, Eliza; Bauman, Melissa D et al. (2017) Early amygdala or hippocampus damage influences adolescent female social behavior during group formation. Behav Neurosci 131:68-82
Seil, Shannon K; Hannibal, Darcy L; Beisner, Brianne A et al. (2017) Predictors of insubordinate aggression among captive female rhesus macaques. Am J Phys Anthropol 164:558-573
Zhang, Xinjun; Kanthaswamy, Sree; Trask, Jessica S et al. (2017) Genetic Characterization of a Captive Colony of Pigtailed Macaques (Macaca nemestrina). J Am Assoc Lab Anim Sci 56:390-395

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