Significance HIV is primarily a sexually transmitted disease but little is known about the transmission of viral variants during sexual contact. An understanding of the types of viruses transmitted by sex may make it possible to design vaccines to prevent the transmission of HIV. Objective In this study we used the rhesus macaque/SIV model to test the hypothesis that specific viral variants are efficiently transmitted by intravenous inoculation but not by vaginal inoculation . Results Five animals were inoculated intravenously with a high dose of SIVmac251. This is a virus stock with that consists of many variants. Five animals were inoculated intravaginally with the same stock. All the animals became infected and we are currrently assessing the natuire of the viral variants that infected the animals. We will compare the variants that infected the IV and IVAG inoculated animals to determine if the route of inoculation influences the type of viruses which infect rhesus macaques. Future Directions If specific SIV variants are responsible for infecting animals after vaginal inoculation, we will serially passage a virus stock by intravaginally inoculating rhesus macaques sequentially. Then we will produce a stock from the plasma of the last animal and use that for vaccine challenge studies. KEYWORDS viral variants, HIV mucosal transmission

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Primate Research Center Grants (P51)
Project #
5P51RR000169-37
Application #
6277935
Study Section
Project Start
1998-05-01
Project End
1999-04-30
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
37
Fiscal Year
1998
Total Cost
Indirect Cost
Name
University of California Davis
Department
Type
DUNS #
094878337
City
Davis
State
CA
Country
United States
Zip Code
95618
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