The Biochemical Core of the proposed TSRI Alcohol Research Center is comprised of twocomponents designed to provide sample analyses and chemical compounds (pharmacological andotherwise) to the Center at large. The biochemical analysis component of the Core will provideanalyses of endocannabinoids (Roberto/Siggins and Parsons components, Taffe pilot project),amino acids (Roberto/Siggins and Parsons components), monoamines (Rivier component andTaffe pilot), CRF (Roberto/Siggins component), NPY (Zorrilla/Weiss and Roberto/Sigginscomponents) and neuroactive steroids (animal models core). These analyses will be performed onin vivo brain microdialysis samples, post-mortem brain tissue, and plasma from rats as well as inprimate CSF using highly sensitive and selective methods including LC-MS, CE-LIF, HPLC-ED andRIA techniques. The Core will also provide consultation regarding optimum sample collection,handling and storage, and will guide experimental design involving in vivo microdialysis techniques.The chemical compound repository component of the Core will provide the necessary standards forthe assays described above as well as for any required internal standards. This component will alsocoordinate the standardization of sensitivity and reproducibility of standard curves used in theseassays. The required CRF, neuropeptide Y, endocannabinoid, amino acid, monoamine, andneurosteroid ligands will also be analyzed for purity as they become available. Ligands whichcannot be obtained commercially will be synthesized as necessary.
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