The CARC's Molecular/Cellular Biology Laboratory Core performs a fundamental role in coordinating the research activities of CARC research components and pilot projects. In addition, the Core will extend its expertise to support NIAAA funded projects that tie to the theme of the CARC. Its mission will be to serve as a core resource for conducting experimental procedures, training technical personnel, and developing cutting-edge analytical techniques that enable our investigators to achieve their CARC research objectives.
The Specific Aims of the Molecular/Cellular Biology Laboratory Core are: 1. To perform cellular phenotyping, functional analysis, and sorting using fluorescent activated cell-sorting (FACS) facilities. 2. To perform molecular biological assays and procedures as needed by the CARC investigators. 3. To perform specific analytical assays for projects supported by the CARC. 4. To maintain a cell culture facility and support research activities utilizing cell culture technology. In addition to the four Specific Aims, the CARC Molecular/Cellular Biology Laboratory Core has an established track record of expanding the repertoire of flow cytometry, gene cloning, Taqman assays, luminex assays, and ELISAs available to CARC investigators. Developmental aspects of the Core will be focused on CARC objectives in order to ensure their succress. These include 1) developing novel Taqman PCR and RT-PCR assays linked to FACS sorting of rare cell events and 2) collaborating with the Geneomics Core Facility at LSUHSC to expand analytical protocols for determination of DNA methylation and chromatin acetylation in the regulation of specific gene expression. Priorities will be enforced to secure maximal productivity of research projects supported by the Center. The personnel in this core will train other personnel and will help coordinate research efforts among projects. The overall goal of the Molecular/Cellular Biology Laboratory Core is to enhance efficiency, quality control, cooperation, and flexibility of research participants in their pursuit of Center objectives.

Public Health Relevance

The Molecular/Cellular Biology Laboratory Core performs a fundamental role in coordinating the investigations of the CARC. Novel information obtained from these studies will greatly advance our knowledge about the adverse effects of alcohol abuse on the development of HIV/AIDS and anti-retroviral therapy treatment of this infection in alcohol abusers

Agency
National Institute of Health (NIH)
Institute
National Institute on Alcohol Abuse and Alcoholism (NIAAA)
Type
Comprehensive Center (P60)
Project #
5P60AA009803-21
Application #
8577111
Study Section
Special Emphasis Panel (ZAA1-DD)
Project Start
Project End
Budget Start
2013-12-01
Budget End
2014-11-30
Support Year
21
Fiscal Year
2014
Total Cost
$212,823
Indirect Cost
$58,014
Name
Louisiana State Univ Hsc New Orleans
Department
Type
DUNS #
782627814
City
New Orleans
State
LA
Country
United States
Zip Code
70112
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Simon, Liz; Siggins, Robert; Winsauer, Peter et al. (2018) Simian Immunodeficiency Virus Infection Increases Blood Ethanol Concentration Duration After Both Acute and Chronic Administration. AIDS Res Hum Retroviruses 34:178-184
Samuelson, Derrick R; Burnham, Ellen L; Maffei, Vincent J et al. (2018) The respiratory tract microbial biogeography in alcohol use disorder. Am J Physiol Lung Cell Mol Physiol 314:L107-L117
Glick, Jennifer L; Theall, Katherine P; Andrinopoulos, Katherine M et al. (2018) The Role of Discrimination in Care Postponement Among Trans-Feminine Individuals in the U.S. National Transgender Discrimination Survey. LGBT Health 5:171-179
Molina, Patricia E; Nelson, Steve (2018) Binge Drinking's Effects on the Body. Alcohol Res 39:99-109
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Ford Jr, Stephen M; Simon Peter, Liz; Berner, Paul et al. (2018) Differential contribution of chronic binge alcohol and antiretroviral therapy to metabolic dysregulation in SIV-infected male macaques. Am J Physiol Endocrinol Metab :
Boule, Lisbeth A; Ju, Cynthia; Agudelo, Marisela et al. (2018) Summary of the 2016 Alcohol and Immunology Research Interest Group (AIRIG) meeting. Alcohol 66:35-43
Glick, Jennifer L; Andrinopoulos, Katherine M; Theall, Katherine P et al. (2018) ""Tiptoeing Around the System"": Alternative Healthcare Navigation Among Gender Minorities in New Orleans. Transgend Health 3:118-126
Glick, Jennifer L; Theall, Katherine; Andrinopoulos, Katherine et al. (2018) For data's sake: dilemmas in the measurement of gender minorities. Cult Health Sex :1-16

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