The Imaging Core has two components that provide major support to the center investigators. The Confocal/Multiphoton Component provides the following: 1) high resolution 3-dimensional imaging of parameter-sensitive fluorophores in living cells and tissues by laser scanning confocal and multiphoton microscopy to monitor ions, electrical potentials, oxygen and nitrogen radical generation, pyridine nucleotide reduction, mitochondfial and plasmalemmal membrane permeability, and cell viability (apoptosis and necrosis); 2) intravital microscopy using """"""""real time"""""""" confocal microscope and multiphoton microscopy to monitor microcirculation, leukocyte margination and gene expression in living animals; 3) high resolution confocal and multiphoton imaging of tissue sections for immunocytochemistry and green fluorescent protein; 4) a digital darkroom facility for digitizing images, analyzing and labeling these images, and printing the processed images as photographic quality prints and slides for use in publications, reports and oral presentations; and 5) a fluorescence plate reader for measuring cell viability and intracellular ions in cultured cells grown on multi-well plates. The Pathology Component provides consultation, training and services in basic histopathology for preparation of frozen and paraffin sections for histology and immunocytochemistry. The Pathology Component routinely handles H&E, PAS, eosin, and trichrome staining of brain, liver and other tissues and provides immunocytochemical staining for various cellular components of interest.

National Institute of Health (NIH)
National Institute on Alcohol Abuse and Alcoholism (NIAAA)
Comprehensive Center (P60)
Project #
Application #
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of North Carolina Chapel Hill
Chapel Hill
United States
Zip Code
Beattie, Matthew C; Reguyal, Christopher S; Porcu, Patrizia et al. (2018) Neuroactive Steroid (3?,5?)3-hydroxypregnan-20-one (3?,5?-THP) and Pro-inflammatory Cytokine MCP-1 Levels in Hippocampus CA1 are Correlated with Voluntary Ethanol Consumption in Cynomolgus Monkey. Alcohol Clin Exp Res 42:12-20
Mazzone, C M; Pati, D; Michaelides, M et al. (2018) Acute engagement of Gq-mediated signaling in the bed nucleus of the stria terminalis induces anxiety-like behavior. Mol Psychiatry 23:143-153
Coleman Jr, Leon G; Crews, Fulton T (2018) Innate Immune Signaling and Alcohol Use Disorders. Handb Exp Pharmacol 248:369-396
Stringfield, Sierra J; Boettiger, Charlotte A; Robinson, Donita L (2018) Nicotine-enhanced Pavlovian conditioned approach is resistant to omission of expected outcome. Behav Brain Res 343:16-20
Harper, Kathryn M; Knapp, Darin J; Criswell, Hugh E et al. (2018) Vasopressin and alcohol: a multifaceted relationship. Psychopharmacology (Berl) 235:3363-3379
Boschen, Karen E; Gong, Henry; Murdaugh, Laura B et al. (2018) Knockdown of Mns1 Increases Susceptibility to Craniofacial Defects Following Gastrulation-Stage Alcohol Exposure in Mice. Alcohol Clin Exp Res 42:2136-2143
Jaramillo, Anel A; Randall, Patrick A; Stewart, Spencer et al. (2018) Functional role for cortical-striatal circuitry in modulating alcohol self-administration. Neuropharmacology 130:42-53
Vetreno, Ryan P; Lawrimore, Colleen J; Rowsey, Pamela J et al. (2018) Persistent Adult Neuroimmune Activation and Loss of Hippocampal Neurogenesis Following Adolescent Ethanol Exposure: Blockade by Exercise and the Anti-inflammatory Drug Indomethacin. Front Neurosci 12:200
Broadwater, Margaret A; Lee, Sung-Ho; Yu, Yang et al. (2018) Adolescent alcohol exposure decreases frontostriatal resting-state functional connectivity in adulthood. Addict Biol 23:810-823
Fiorenza, Amanda M; Shnitko, Tatiana A; Sullivan, Kaitlin M et al. (2018) Ethanol Exposure History and Alcoholic Reward Differentially Alter Dopamine Release in the Nucleus Accumbens to a Reward-Predictive Cue. Alcohol Clin Exp Res 42:1051-1061

Showing the most recent 10 out of 227 publications