The Basic Science and Engineering Core facility will make the following services available to the Division on Aging: 1) A Genome Scanning for DNA Markers Service will assist the geriatrician investigator with the identification of genomic sequences and DNA marker panels that are associated with the prevalent disease of late life. Specialized 2-D electrophoresis units will be provided. 2) A gene Identification and Characterization Services will enable the geriatrician/gerontologist to identify genes that are differentially expressed during aging and/or disease. Equipment for subtractive hybridization and PCR differential display techniques will be available. 3) An Oxidative DNA Damage Determination Service will allow the clinician investigator to determine the extent of oxidative DNA damage that is detectable in the blood or urine in a given patient. High performance liquid chromatography analysis will be available. 4) A Transgenic Animal Design Service will assist investigators in the design of transgenic mice using microinjection of DNA or gene targeting and homologous recombination. Access to the microinjection equipment or embryonic stem cell laboratory will be provided. 5) A Biological Data Acquisition Service provides recording devices for walking cadence, ECG and continuous blood pressure. It also loans transducers, amplifiers, and recorders for the monitoring of biological signals. 6) A Biological Signal Processing Service digitizes, displays and analyzes biological signals such as those obtained through service #5. Special software for the analysis of walking cadence, ECG, and blood pressure as well as general purpose software (for performing Fourier transforms, filtering, principal component analysis, and for calculating non-linear diagnostics such as Lyapunov exponents) will be provided. 7) An Image Acquisition Service provides research grade microscopy for cell biology projects that require high resolution imaging. This service also makes available video cameras and recorders for the study of patient gait and movement. 8) An Image Processing and Interpretation Service performs digital image processing of images such as those recorded by the image acquisition service, including image enhancement and filtering, image segmentation and pattern recognition, and 3-D reconstruction from microscopic optical serial sections.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Comprehensive Center (P60)
Project #
3P60AG008812-10S1
Application #
6314525
Study Section
Project Start
1999-09-30
Project End
2001-02-28
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
10
Fiscal Year
1999
Total Cost
Indirect Cost
Name
Harvard University
Department
Type
DUNS #
082359691
City
Boston
State
MA
Country
United States
Zip Code
02115
Foley, Jessica M; Salat, David H; Stricker, Nikki H et al. (2016) Glucose Dysregulation Interacts With APOE-?4 to Potentiate Temporoparietal Cortical Thinning. Am J Alzheimers Dis Other Demen 31:76-86
Stricker, Nikki H; Salat, David H; Kuhn, Taylor P et al. (2016) Mild Cognitive Impairment is Associated With White Matter Integrity Changes in Late-Myelinating Regions Within the Corpus Callosum. Am J Alzheimers Dis Other Demen 31:68-75
Fredman, Lisa; Gordon, Sheryl A; Heeren, Timothy et al. (2014) Positive affect is associated with fewer sleep problems in older caregivers but not noncaregivers. Gerontologist 54:559-69
Foley, Jessica M; Salat, David H; Stricker, Nikki H et al. (2014) Interactive effects of apolipoprotein E4 and diabetes risk on later myelinating white matter regions in neurologically healthy older aged adults. Am J Alzheimers Dis Other Demen 29:222-35
Leritz, Elizabeth C; Shepel, Juli; Williams, Victoria J et al. (2014) Associations between T1 white matter lesion volume and regional white matter microstructure in aging. Hum Brain Mapp 35:1085-100
Morley, John E (2013) Systolic hypertension should not be treated in persons aged 80 and older until blood pressure is greater than 160 mmHg. J Am Geriatr Soc 61:1197-8
Jones, Richard N; Marsiske, Michael; Ball, Karlene et al. (2013) The ACTIVE cognitive training interventions and trajectories of performance among older adults. J Aging Health 25:186S-208S
Stricker, Nikki H; Salat, David H; Foley, Jessica M et al. (2013) Decreased white matter integrity in neuropsychologically defined mild cognitive impairment is independent of cortical thinning. J Int Neuropsychol Soc 19:925-37
Williams, Victoria J; Leritz, Elizabeth C; Shepel, Juli et al. (2013) Interindividual variation in serum cholesterol is associated with regional white matter tissue integrity in older adults. Hum Brain Mapp 34:1826-41
Salat, David H; Williams, Victoria J; Leritz, Elizabeth C et al. (2012) Inter-individual variation in blood pressure is associated with regional white matter integrity in generally healthy older adults. Neuroimage 59:181-92

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