The DNA sequencing core facility has been an important component of the NEOMAMDC since 1993, and has significantly enhanced the research infrastructure of the Center, and accelerated progress for Arthritis researchers. Although the scope of molecular biology procedures used by laboratories associated with the Arthritis Center is quite broad and are project-specific most laboratories of the NEOMAMDC have benefitted and will continue to benefit from a core facility dedicated to DNA sequence determination. The rationale for this is that 1) DNA sequencing is required at some point in any molecular biology oriented experimental approach, and 2) it is not always cost effective for each laboratory to set up individual protocols, purchase equipment an train the necessary personnel. 3) DNA sequence analysis is labor and time intensive, but does not require specific knowledge of the large project. 4) The on site sequencing facility enhances the overall productivity of Arthritis Center research laboratories by performing this task more efficiently and with greater precision than would be possible on an individual basis 5) The core facility frees time for investigators to engage in more creative pursuits. The primary objective of the core is to assist those investigators already engaged in molecular biology protocols. In order to be practical, it does not serve to perform any molecular biology technique other than DNA sequence analysis. Investigators are responsible for preparation of samples for sequencing in advance of submission. Protocols for samples preparation are provided by the sequencing core in order to assure that the core facility will be efficiently utilize and to assure success. In general, investigators are responsible for ligation of DNA fragments into the polylinker (cloning site) of sequencing vectors commonly in use. The core facility is then responsible for performing the sequencing reactions, running the automated sequencing instrument and recording the sequence as a file that can be used for further analysis by the investigator. The second objective of the core is to serve as repository for genomic DNA from members of families afflicted with OA showing familial inheritance, and from patients with OA disease subsets. DNA from these individuals is made available to other investigators on a collaborative basis. Additionally, the core functions to screen this DNA bank for mutations in the type II collagen gene.

Project Start
2001-07-01
Project End
2003-06-30
Budget Start
Budget End
Support Year
23
Fiscal Year
2001
Total Cost
$38,258
Indirect Cost
Name
Case Western Reserve University
Department
Type
DUNS #
077758407
City
Cleveland
State
OH
Country
United States
Zip Code
44106
Miwa, Hazuki E; Gerken, Thomas A; Huynh, Tru D et al. (2009) Conserved sequence in the aggrecan interglobular domain modulates cleavage by ADAMTS-4 and ADAMTS-5. Biochim Biophys Acta 1790:161-72
Atif, U; Philip, A; Aponte, J et al. (2008) Absence of association of asporin polymorphisms and osteoarthritis susceptibility in US Caucasians. Osteoarthritis Cartilage 16:1174-7
Kraus, V B; Jordan, J M; Doherty, M et al. (2007) The Genetics of Generalized Osteoarthritis (GOGO) study: study design and evaluation of osteoarthritis phenotypes. Osteoarthritis Cartilage 15:120-7
Miwa, Hazuki E; Gerken, Thomas A; Huynh, Tru D et al. (2006) Mammalian expression of full-length bovine aggrecan and link protein: formation of recombinant proteoglycan aggregates and analysis of proteolytic cleavage by ADAMTS-4 and MMP-13. Biochim Biophys Acta 1760:472-86
Ievers-Landis, Carolyn E; Burant, Christopher; Drotar, Dennis et al. (2005) A randomized controlled trial for the primary prevention of osteoporosis among preadolescent girl scouts: 1-year outcomes of a behavioral program. J Pediatr Psychol 30:155-65
Vashishth, D (2005) Collagen glycation and its role in fracture properties of bone. J Musculoskelet Neuronal Interact 5:316
Denko, Charles W; Malemud, Charles J (2005) Serum growth hormone and insulin but not insulin-like growth factor-1 levels are elevated in patients with fibromyalgia syndrome. Rheumatol Int 25:146-51
Holderbaum, D; Malvitz, T; Ciesielski, C J et al. (2005) A newly described hereditary cartilage debonding syndrome. Arthritis Rheum 52:3300-4
Denko, Charles W; Malemud, Charles J (2005) Role of the growth hormone/insulin-like growth factor-1 paracrine axis in rheumatic diseases. Semin Arthritis Rheum 35:24-34
Denko, Charles W; Malemud, Charles J (2004) Age-related changes in serum growth hormone, insulin-like growth factor-1 and somatostatin in system lupus erythematosus. BMC Musculoskelet Disord 5:37

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