Abstract

A major part of the Center's overall research base employs genetic based studies of rheumatic disease. The techniques supported by this proposed molecular genotyping and sequencing core are central to the evaluation of these disease populations. The core will provide support in the following areas; dideoxysequencing of PCR products or inserts cloned in vectors; genotype analysis using microsatellite markers that define polymorphisms throughout the genome; and typing of disease-related polymorphisms including HLA and TCR genes. The core will maintain and coordinate use of a semiautomated DNA sequencer which be available for both sequencing and genome scans for gene polymorphisms. The molecular genotyping and sequencing core will also aid in the interpretation of DNA sequence information as well as maintain current technologies. A molecular genotyping and sequencing core is not available in any other department within the institution.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Comprehensive Center (P60)
Project #
1P60AR044059-01
Application #
5206381
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
1996
Total Cost
Indirect Cost

  Publications

Brunner, Hermine I; Klein-Gitelman, Marisa S; Zelko, Frank et al. (2013) Validation of the Pediatric Automated Neuropsychological Assessment Metrics in childhood-onset systemic lupus erythematosus. Arthritis Care Res (Hoboken) 65:372-81
Wallace, Carol A; Giannini, Edward H; Huang, Bin et al. (2011) American College of Rheumatology provisional criteria for defining clinical inactive disease in select categories of juvenile idiopathic arthritis. Arthritis Care Res (Hoboken) 63:929-36
Suzuki, Michiko; Wiers, Kristina; Brooks, Elizabeth B et al. (2009) Initial validation of a novel protein biomarker panel for active pediatric lupus nephritis. Pediatr Res 65:530-6
Carrasco, Ruy; Lovell, Daniel J; Giannini, Edward H et al. (2008) Biochemical markers of bone turnover associated with calcium supplementation in children with juvenile rheumatoid arthritis: results of a double-blind, placebo-controlled intervention trial. Arthritis Rheum 58:3932-40
Lovell, Daniel J; Glass, David; Ranz, Julie et al. (2006) A randomized controlled trial of calcium supplementation to increase bone mineral density in children with juvenile rheumatoid arthritis. Arthritis Rheum 54:2235-42
Morris, Suzanne C; Dragula, Nanette L; Finkelman, Fred D (2002) IL-4 promotes Stat6-dependent survival of autoreactive B cells in vivo without inducing autoantibody production. J Immunol 169:1696-704
Dangoria, Nandita S; DeLay, Monica L; Kingsbury, Daniel J et al. (2002) HLA-B27 misfolding is associated with aberrant intermolecular disulfide bond formation (dimerization) in the endoplasmic reticulum. J Biol Chem 277:23459-68
Yang, Lin; Thornton, Sherry; Grom, Alexei A (2002) Interleukin-15 inhibits sodium nitroprusside-induced apoptosis of synovial fibroblasts and vascular endothelial cells. Arthritis Rheum 46:3010-4
Strait, Richard T; Morris, Suzanne C; Yang, Mingyan et al. (2002) Pathways of anaphylaxis in the mouse. J Allergy Clin Immunol 109:658-68
Gylys-Morin, V M; Graham, T B; Blebea, J S et al. (2001) Knee in early juvenile rheumatoid arthritis: MR imaging findings. Radiology 220:696-706

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