Abstract

The Cell Imaging Subcore was started in September, 1993. The objective of this subcore is to provide access to, and expert assistance in, the use of a state-of-the-art laser scanning confocal microscope. Advice is available on immunostaining, dynamic fluorescence imaging, and computer-based image processing. The key resource of this subcore is a Zeiss LSM410 confocal microscope equipped with an external Ar-Kr laser. Four Silicon Graphics workstations equipped with image analysis software support the analysis and visualization of the large and complex data sets generated by the confocal microscope. Researchers who use this resource receive assistance with three-dimensional image processing, visualization, and hard copy output. In the presubmission survey of DRTC members, 22% indicated that they anticipated extensive use of this subcore. At least seven DRTC investigators currently use this subcore extensively for diabetes-related studies.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Comprehensive Center (P60)
Project #
5P60DK020593-20
Application #
6270501
Study Section
Project Start
1998-01-20
Project End
1998-11-30
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
20
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
Vanderbilt University Medical Center
Department
Type
DUNS #
004413456
City
Nashville
State
TN
Country
United States
Zip Code
37212

  Publications

Wilson, Christopher S; Chhabra, Preeti; Marshall, Andrew F et al. (2018) Healthy Donor Polyclonal IgMs Diminish B-Lymphocyte Autoreactivity, Enhance Regulatory T-Cell Generation, and Reverse Type 1 Diabetes in NOD Mice. Diabetes 67:2349-2360
Hughey, Curtis C; Trefts, Elijah; Bracy, Deanna P et al. (2018) Glycine N-methyltransferase deletion in mice diverts carbon flux from gluconeogenesis to pathways that utilize excess methionine cycle intermediates. J Biol Chem 293:11944-11954
Yao, Lina; Seaton, Sarah Craven; Ndousse-Fetter, Sula et al. (2018) A selective gut bacterial bile salt hydrolase alters host metabolism. Elife 7:
Mani, Bharath K; Castorena, Carlos M; Osborne-Lawrence, Sherri et al. (2018) Ghrelin mediates exercise endurance and the feeding response post-exercise. Mol Metab 9:114-130
Bolus, W Reid; Peterson, Kristin R; Hubler, Merla J et al. (2018) Elevating adipose eosinophils in obese mice to physiologically normal levels does not rescue metabolic impairments. Mol Metab 8:86-95
West, Kathryn L; Kelm, Nathaniel D; Carson, Robert P et al. (2018) Myelin volume fraction imaging with MRI. Neuroimage 182:511-521
Barke, Theresa L; Goldstein, Jeffery A; Sundermann, Alexandra C et al. (2018) Gestational diabetes mellitus is associated with increased CD163 expression and iron storage in the placenta. Am J Reprod Immunol 80:e13020
Rohrbough, Jeffrey; Nguyen, Hong-Ngan; Lamb, Fred S (2018) Modulation of ClC-3 gating and proton/anion exchange by internal and external protons and the anion selectivity filter. J Physiol 596:4091-4119
Schlegel, Cameron; Lapierre, Lynne A; Weis, Victoria G et al. (2018) Reversible deficits in apical transporter trafficking associated with deficiency in diacylglycerol acyltransferase. Traffic 19:879-892
Moore, Mary Courtney; Smith, Marta S; Farmer, Ben et al. (2018) Morning Hyperinsulinemia Primes the Liver for Glucose Uptake and Glycogen Storage Later in the Day. Diabetes 67:1237-1245

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