Abstract

The Transgenic Mouse/ES Cell subcore is a new support laboratory that is jointly administered by the DRTC and the Vanderbilt Cancer Center. The objective of this subcore is to assist investigators in generating germline-altered mice. These animals provide useful models for the study of diabetes and other diseases. Two key services are offered: the generation of transgenic mice by the microinjection of DNA and the production of chimeric mice by the microinjection of embryonic stem (ES) cells. Personnel of this subcore provide consultation in the generation and maintenance of transgenic mice. The subcore has been in operation for over a year and a half and has produced over 100 transgenic founder animals by the pronuclear microinjection of DNA. It has also aided investigators in performing gene knockout experiments by generating chimeric mice. An ES cell laboratory that will assist investigators in the performance of gene knockout experiments is being established as part of this subcore. This subcore met with high user approval in a presubmission review of our service facilities. Nine DRTC investigators intend to use this facility in 1995-96 for either pronuclear DNA microinjections or for embryonic stem cell injections into blastocysts.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Comprehensive Center (P60)
Project #
3P60DK020593-21S1
Application #
6105108
Study Section
Project Start
1999-04-15
Project End
1999-11-30
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
21
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
Vanderbilt University Medical Center
Department
Type
DUNS #
004413456
City
Nashville
State
TN
Country
United States
Zip Code
37212

  Publications

Wilson, Christopher S; Chhabra, Preeti; Marshall, Andrew F et al. (2018) Healthy Donor Polyclonal IgMs Diminish B-Lymphocyte Autoreactivity, Enhance Regulatory T-Cell Generation, and Reverse Type 1 Diabetes in NOD Mice. Diabetes 67:2349-2360
Hughey, Curtis C; Trefts, Elijah; Bracy, Deanna P et al. (2018) Glycine N-methyltransferase deletion in mice diverts carbon flux from gluconeogenesis to pathways that utilize excess methionine cycle intermediates. J Biol Chem 293:11944-11954
Yao, Lina; Seaton, Sarah Craven; Ndousse-Fetter, Sula et al. (2018) A selective gut bacterial bile salt hydrolase alters host metabolism. Elife 7:
Mani, Bharath K; Castorena, Carlos M; Osborne-Lawrence, Sherri et al. (2018) Ghrelin mediates exercise endurance and the feeding response post-exercise. Mol Metab 9:114-130
Bolus, W Reid; Peterson, Kristin R; Hubler, Merla J et al. (2018) Elevating adipose eosinophils in obese mice to physiologically normal levels does not rescue metabolic impairments. Mol Metab 8:86-95
West, Kathryn L; Kelm, Nathaniel D; Carson, Robert P et al. (2018) Myelin volume fraction imaging with MRI. Neuroimage 182:511-521
Barke, Theresa L; Goldstein, Jeffery A; Sundermann, Alexandra C et al. (2018) Gestational diabetes mellitus is associated with increased CD163 expression and iron storage in the placenta. Am J Reprod Immunol 80:e13020
Rohrbough, Jeffrey; Nguyen, Hong-Ngan; Lamb, Fred S (2018) Modulation of ClC-3 gating and proton/anion exchange by internal and external protons and the anion selectivity filter. J Physiol 596:4091-4119
Schlegel, Cameron; Lapierre, Lynne A; Weis, Victoria G et al. (2018) Reversible deficits in apical transporter trafficking associated with deficiency in diacylglycerol acyltransferase. Traffic 19:879-892
Moore, Mary Courtney; Smith, Marta S; Farmer, Ben et al. (2018) Morning Hyperinsulinemia Primes the Liver for Glucose Uptake and Glycogen Storage Later in the Day. Diabetes 67:1237-1245

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