While in vivo methods have proven invaluable in determining the physiological characteristics of human disease, progress in understanding rodent models has been delayed due to the lack of methods. Rapid progress in miniaturization, and in the increased sensitivity of monitoring devices has provided tools that can access information from relatively undisturbed rodents. The Animal Physiology Core (APC) brings together four experts in the areas of integrative physiology, cardiovascular physiology, imaging, and animal models to serve as a core resource for the study of diabetes. The goal of the APC is to facilitate and enhance the combined metabolic and vascular phenotyping of small animals by providing easy access to highly-specialized equipment and expertise in the areas of body composition, energetics, glucose homeostasis, cardiovascular assessment, molecular imaging, and transgenic animal models. This will enhance the capability and cost-effectiveness for DRTC members in the use of small animals for studying diabetes, diabetes complications, and cardiometabolic disease.
The specific aims of the Core are to provide the following: 1 Body composition: Whole-body composition analysis by chemical carcass analysis, dual-energy X-ray absorptiometry (DXA), quantitative magnetic resonance (QMR), and micro-computed tomography. 2 Energy balance: Comprehensive assessments of metabolic rate (indirect calorimetry), food intake, fecal output, activity, and body temperature. 3 Glucose homeostasis: Glucose and insulin tolerance testing and hyperinsulinemic clamps. 4 Cardiovascular assessment: Echocardiography and myocardial function, vascular compliance, and blood pressure. 5 Metabolic/Molecular Imaging: Bioluminescence, fluorescence, and gamma-ray imaging, including SPECT/CT. 6 Transgenic animal models: Assistance with construct preparation, generation of transgenic/knock-out mice, husbandry and colony management, and genotyping. These services are based upon the expressed needs of DRTC investigators, and will incorporate expertise and current technology for both metabolism and vascular assessment, brought together in one core for combined applications by the user base. This will facilitate a more sophisticated, multidisciplinary,and comprehensive approach to diabetes research, and provide common ground for collaboration

National Institute of Health (NIH)
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Comprehensive Center (P60)
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Special Emphasis Panel (ZDK1-GRB-S (O1))
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University of Alabama Birmingham
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Kang, Minsung; Liu, Xiaobing; Fu, Yuchang et al. (2018) Improved systemic metabolism and adipocyte biology in miR-150 knockout mice. Metabolism 83:139-148
Jo, SeongHo; Chen, Junqin; Xu, Guanlan et al. (2018) miR-204 Controls Glucagon-Like Peptide 1 Receptor Expression and Agonist Function. Diabetes 67:256-264
Mohler 3rd, Emile R; Ellenberg, Susan S; Lewis, Cora E et al. (2018) The Effect of Testosterone on Cardiovascular Biomarkers in the Testosterone Trials. J Clin Endocrinol Metab 103:681-688
Hunter, Gary R; Bryan, David R; Borges, Juliano H et al. (2018) Racial Differences in Relative Skeletal Muscle Mass Loss During Diet-Induced Weight Loss in Women. Obesity (Silver Spring) 26:1255-1260
Wingo, Brooks C; Barry, Valene Garr; Ellis, Amy C et al. (2018) Comparison of segmental body composition estimated by bioelectrical impedance analysis and dual-energy X-ray absorptiometry. Clin Nutr ESPEN 28:141-147
Hunter, Gary R; Plaisance, Eric P; Carter, Stephen J et al. (2018) Why intensity is not a bad word: Optimizing health status at any age. Clin Nutr 37:56-60
Engle, Staci E; Antonellis, Patrick J; Whitehouse, Logan S et al. (2018) A CreER mouse to study melanin concentrating hormone signaling in the developing brain. Genesis 56:e23217
Hunter, Gary R; Fisher, Gordon; Bryan, David R et al. (2018) Divergent Blood Pressure Response After High-Intensity Interval Exercise: A Signal of Delayed Recovery? J Strength Cond Res 32:3004-3010
Snyder, Peter J; Bhasin, Shalender; Cunningham, Glenn R et al. (2018) Lessons From the Testosterone Trials. Endocr Rev 39:369-386
Ingram, K H; Hunter, G R; James, J F et al. (2017) Central fat accretion and insulin sensitivity: differential relationships in parous and nulliparous women. Int J Obes (Lond) 41:1214-1217

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