Core - Laboratory

Moore, R Blaine

Abstract

The Comprehensive Sickle Cell Center is composed of several basic, clinical and developmental research projects which can benefit from a centralized Laboratory Core unit. The major objectives of the laboratory are to act as the liaison between the clinical coordinator of the Administrative Core and the project investigators to obtain blood and other samples, to provide services which are common to several research projects and to facilitate shipping and storage of samples. This will be accomplished through the following Specific goals: 1. coordinating the requests and distribution of blood samples for project investigators; 2. providing hematologic, microscopic, nutritional, biochemical and molecular genetic information on sickle cell subjects from which research blood is obtained; 3. maintaining a multi-user equipment room for the basic and clinical research projects; 4. organizing the long term cryogenic storage of plasma and other blood products; 5. assisting the Centralized Pathology Unit for Sickle Cell disease; and 6. coordinating the receipt and shipment of packages which are coming from or going to other research centers or institutions.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Heart, Lung, and Blood Institute (NHLBI)
• Type: Comprehensive Center (P60)
• Project #: 2P60HL038639-06
• Application #: 3780649
• Support Year: 6
• Fiscal Year: 1993

Institution

• Name: University of South Alabama
• City: Mobile
• State: AL
• Country: United States
• Zip Code: 36688

Publications

Haynes Jr, Johnson; Baliga, B Surendra; Obiako, Boniface et al. (2004) Zileuton induces hemoglobin F synthesis in erythroid progenitors: role of the L-arginine-nitric oxide signaling pathway. Blood 103:3945-50

Pace, B S; Qian, X; Ofori-Acquah, S F (2004) Selective inhibition of beta-globin RNA transcripts by antisense RNA molecules. Cell Mol Biol (Noisy-le-grand) 50:43-51

Haynes Jr, Johnson; Obiako, Boniface (2002) Activated polymorphonuclear cells increase sickle red blood cell retention in lung: role of phospholipids. Am J Physiol Heart Circ Physiol 282:H122-30

Foley, Heather A; Ofori-Acquah, Solomon F; Yoshimura, Akihiko et al. (2002) Stat3 beta inhibits gamma-globin gene expression in erythroid cells. J Biol Chem 277:16211-9

Abraham, Ann; Bencsath, F Aladar; Shartava, Archil et al. (2002) Preparation of irreversibly sickled cell beta-actin from normal red blood cell beta-actin. Biochemistry 41:292-6

Kakhniashvili, D G; Goodman, S R (2001) Isolation of spectrin subunits by reverse-phase high-performance liquid chromatography. Protein Expr Purif 23:249-51

Sangerman, J; Kakhniashvili, D; Brown, A et al. (2001) Spectrin ubiquitination and oxidative stress: potential roles in blood and neurological disorders. Cell Mol Biol Lett 6:607-36

Yang, Y M; Pace, B (2001) Pharmacologic induction of fetal hemoglobin synthesis: cellular and molecular mechanisms. Pediatr Pathol Mol Med 20:87-106

Cepeda, M L; Allen, F H; Cepeda, N J et al. (2000) Physical growth, sexual maturation, body image and sickle cell disease. J Natl Med Assoc 92:4-Oct

Xu, L; Ferry, A E; Monteiro, C et al. (2000) Beta globin gene inhibition by antisense RNA transcripts. Gene Ther 7:438-44