Abstract

The overall aims of the core are to perform diagnostic test for sickle cell anemia and thalassemia at the DNA level and to provide molecular biology support to investigators of the center. The core will perform sickle cell anemia, diagnostic, alpha- thalassemia (alphaSEA, alphaMED, alpha20.5), HPFH-1, HPFH-2, and haplotypes determination using the Polymerase Chain Reaction. Diagnostic thalassemia alpha3.7 and alpha4.2 deletions will be performed by Southern blot after digestion by BamHI. In addition, the core will help investigators of the center perform genomic DNA and RNA isolation, plasmid constructions, transfections and analysis of gene expression using reporter assay, primer extension and FACSGAL analysis. In addition the core will serve as an interface between center investigators and the core facilities of the Albert Einstein College of Medicine (Oligonucleotides and DNA sequencing, transgenic mice and knock-out production etc.)

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Comprehensive Center (P60)
Project #
5P60HL038655-13
Application #
6325948
Study Section
Project Start
2000-04-01
Project End
2001-03-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
13
Fiscal Year
2000
Total Cost
$121,557
Indirect Cost

  Institution

Name
Albert Einstein College of Medicine
Department
Type
DUNS #
009095365
City
Bronx
State
NY
Country
United States
Zip Code
10461

  Publications

Olivier, Emmanuel N; Rybicki, Anne C; Bouhassira, Eric E (2006) Differentiation of human embryonic stem cells into bipotent mesenchymal stem cells. Stem Cells 24:1914-22
Srinivasulu, Sonati; Perumalsamy, Krishnaveni; Upadhya, Rajendra et al. (2006) Pair-wise interactions of polymerization inhibitory contact site mutations of hemoglobin-S. Protein J 25:503-16
Oh, Il-Hoan; Fabry, Mary E; Humphries, R Keith et al. (2004) Expression of an anti-sickling beta-globin in human erythroblasts derived from retrovirally transduced primitive normal and sickle cell disease hematopoietic cells. Exp Hematol 32:461-9
Kaul, Dhananjay K; Liu, Xiao-du; Chang, Hee-Yoon et al. (2004) Effect of fetal hemoglobin on microvascular regulation in sickle transgenic-knockout mice. J Clin Invest 114:1136-45
Kaul, Dhananjay K; Fabry, Mary E (2004) In vivo studies of sickle red blood cells. Microcirculation 11:153-65
Romero, Jose R; Suzuka, Sandra M; Nagel, Ronald L et al. (2004) Expression of HbC and HbS, but not HbA, results in activation of K-Cl cotransport activity in transgenic mouse red cells. Blood 103:2384-90
Wang, Jian-Ying; Drlica, Karl (2003) Modeling hybridization kinetics. Math Biosci 183:37-47
Alami, Raouf; Fan, Yuhong; Pack, Stephanie et al. (2003) Mammalian linker-histone subtypes differentially affect gene expression in vivo. Proc Natl Acad Sci U S A 100:5920-5
Miller, Cindy L; Imren, Suzan; Antonchuk, Jennifer et al. (2002) Feasibility of using autologous transplantation to evaluate hematopoietic stem cell-based gene therapy strategies in transgenic mouse models of human disease. Mol Ther 6:422-8
Lutty, Gerard A; Otsuji, Tsuyoshi; Taomoto, Makoto et al. (2002) Mechanisms for sickle red blood cell retention in choroid. Curr Eye Res 25:163-71

Showing the most recent 10 out of 143 publications