(application abstract) We propose to create an NDC for the Optical Control of Biological Function, to develop methods for rapidly turning select proteins in cells on and off with light. The strategies are broadly applicable across protein classes. The approach will be used for the non-invasive, quantitatively precise, control of the biomolecular dynamics of individual proteins, and for controlling signaling interactions between pairs of proteins. This approach can bring us closer to one of the central goals in biology: that of directly relating molecular events in particular cells to the function of an organ and the behavior of the organism. The developments hold promise in medicine for drug discovery, synthetic biology and the direct treatment of disease. We will develop three technology platforms: 1) chemical and molecular toolkits of modular parts for optical control and chemical integration into proteins, 2) viral delivery for installing photo-switchable proteins into cells, and 3) light delivery systems that make it possible to address these nanodvices in vivo. We will work to solve three engineering and clinical challenges: a) devise a general strategy for optical control over the functional state of individual proteins and the signaling interactions of pairs or groups of proteins;b) gain optical control over the signaling and enzymatic activity of cells in vitro, and c) adapt these approaches to treat retinal and cardiac pathologies. Members of the NDC will be organized into integrated, interdisciplinary teams. We expect three major outcomes. First, rounds of chemical synthesis, genetic engineering and biological testing will help develop the basic design for optical control of protein function, for the specific orthogonal attachment of light-gates, and for the optimal methods of molecular and light delivery in vivo. Second, we will employ light-gated proteins in animal models of human disease. Third, over 5 years we will formulate strategies for applications to cell-based drug screening and remote control switches for synthetic cells, and we will evaluate toxicity to guide the development of biocompatible devices that could be used in the clinic.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Development Center (PN2)
Project #
3PN2EY018241-03S1
Application #
7777536
Study Section
Special Emphasis Panel (ZEY1-VSN (13))
Program Officer
Fisher, Richard S
Project Start
2006-09-30
Project End
2011-09-29
Budget Start
2008-09-30
Budget End
2009-09-29
Support Year
3
Fiscal Year
2009
Total Cost
$500,000
Indirect Cost
Name
University of California Berkeley
Department
Biochemistry
Type
Schools of Arts and Sciences
DUNS #
124726725
City
Berkeley
State
CA
Country
United States
Zip Code
94704
Neely, Aaron M; Zhao, Guoping; Schwarzer, Christian et al. (2018) N-(3-Oxo-acyl)-homoserine lactone induces apoptosis primarily through a mitochondrial pathway in fibroblasts. Cell Microbiol 20:
Xiao, Tong; Ackerman, Cheri M; Carroll, Elizabeth C et al. (2018) Copper regulates rest-activity cycles through the locus coeruleus-norepinephrine system. Nat Chem Biol 14:655-663
Newman, Zachary L; Hoagland, Adam; Aghi, Krishan et al. (2017) Input-Specific Plasticity and Homeostasis at the Drosophila Larval Neuromuscular Junction. Neuron 93:1388-1404.e10
Kienzler, Michael A; Isacoff, Ehud Y (2017) Precise modulation of neuronal activity with synthetic photoswitchable ligands. Curr Opin Neurobiol 45:202-209
Palty, Raz; Fu, Zhu; Isacoff, Ehud Y (2017) Sequential Steps of CRAC Channel Activation. Cell Rep 19:1929-1939
Berry, Michael H; Holt, Amy; Levitz, Joshua et al. (2017) Restoration of patterned vision with an engineered photoactivatable G protein-coupled receptor. Nat Commun 8:1862
Pantoja, Carlos; Hoagland, Adam; Carroll, Elizabeth et al. (2017) Measuring Behavioral Individuality in the Acoustic Startle Behavior in Zebrafish. Bio Protoc 7:
Tochitsky, Ivan; Trautman, Jay; Gallerani, Nicholas et al. (2017) Restoring visual function to the blind retina with a potent, safe and long-lasting photoswitch. Sci Rep 7:45487
Levitz, Joshua; Broichhagen, Johannes; Leippe, Philipp et al. (2017) Dual optical control and mechanistic insights into photoswitchable group II and III metabotropic glutamate receptors. Proc Natl Acad Sci U S A 114:E3546-E3554
Zhao, Guoping; Neely, Aaron M; Schwarzer, Christian et al. (2016) N-(3-oxo-acyl) homoserine lactone inhibits tumor growth independent of Bcl-2 proteins. Oncotarget 7:5924-42

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