Abstract

The basic objective of this study is to determine what effect dietary restriction has on the transfer of genetic information throughout the life span of an organism. In this study, male Fischer F 344 rats will be fed a laboratory chow diet ad libitum or sixty percent of ad libitum (restricted diet). The mean and maximum survival of these two groups of rats will be established. Periodically, the translational and transcriptional activities of several tissues from rats on the two dietary regimens will be determined. Using the post-mitochondrial supernatant from brain and kidney tissue and isolated hepatocytes, protein synthesis will be measured by the incorporation of radioactively-labeled L-valine into acid-insoluble material to determine the translational activities of these tissues from rats in this study. The transcriptioal activity of liver will be ascertained by measuring RNA synthesis by the ability of suspensions of hepatocytes to incorporate radioactively-labeled orotic acid-insoluble material. From this research, it will be possible to determine whether the increased longevity associated with dietary restriction is accompanied by a decrease in the synthesis of RNA or protein and/or a delay in the age-related changes in the synthesis of RNA or protein.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Project (R01)
Project #
5R01AG001548-07
Application #
3114222
Study Section
(SSS)
Project Start
1979-07-01
Project End
1990-06-30
Budget Start
1989-07-01
Budget End
1990-06-30
Support Year
7
Fiscal Year
1989
Total Cost
Indirect Cost

  Institution

Name
Illinois State University
Department
Type
Schools of Arts and Sciences
DUNS #
City
Normal
State
IL
Country
United States
Zip Code
61790

  Publications

Heydari, A R; You, S; Takahashi, R et al. (2000) Age-related alterations in the activation of heat shock transcription factor 1 in rat hepatocytes. Exp Cell Res 256:83-93
Gutsmann-Conrad, A; Pahlavani, M A; Heydari, A R et al. (1999) Expression of heat shock protein 70 decreases with age in hepatocytes and splenocytes from female rats. Mech Ageing Dev 107:255-70
Moore, S A; Lopez, A; Richardson, A et al. (1998) Effect of age and dietary restriction on expression of heat shock protein 70 in rat alveolar macrophages. Mech Ageing Dev 104:59-73
Van Remmen, H; Williams, M D; Yang, H et al. (1998) Analysis of the transcriptional activity of the 5'-flanking region of the rat catalase gene in transiently transfected cells and in transgenic mice. J Cell Physiol 174:18-26
Guo, Z M; Van Remmen, H; Wu, W T et al. (1998) Effect of cAMP-induced transcription on the repair of the phosphoenolpyruvate carboxykinase gene by hepatocytes isolated from young and old rats. Mutat Res 409:37-48
Guo, Z; Heydari, A R; Wu, W et al. (1998) Characterization of gene-specific DNA repair by primary cultures of rat hepatocytes. J Cell Physiol 176:314-22
Gutsmann-Conrad, A; Heydari, A R; You, S et al. (1998) The expression of heat shock protein 70 decreases with cellular senescence in vitro and in cells derived from young and old human subjects. Exp Cell Res 241:404-13
Pahlavani, M A; Harris, M D; Richardson, A (1997) The increase in the induction of IL-2 expression with caloric restriction is correlated to changes in the transcription factor NFAT. Cell Immunol 180:10-9
Pahlavani, M A; Harris, M D; Moore, S A et al. (1996) Expression of heat shock protein 70 in rat spleen lymphocytes is affected by age but not by food restriction. J Nutr 126:2069-75
Heydari, A R; You, S; Takahashi, R et al. (1996) Effect of caloric restriction on the expression of heat shock protein 70 and the activation of heat shock transcription factor 1. Dev Genet 18:114-24

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