Studies of an Inducible Bacterial Enzyme

Zabin, Irving

Abstract

The structure of beta-galactosidase of the Lactose Operon in Escherichia coli will be probed with antibodies, by proteolysis, and by complementation using fragments of the protein. Hybrid proteins containing enzymatically active beta-galactosidase will be examined. The amino acid sequence of thiogalactoside transacetylase will be compared to that of beta-galactosidase.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Allergy and Infectious Diseases (NIAID)
• Type: Research Project (R01)
• Project #: 5R01AI004181-24
• Application #: 3124182
• Study Section: Microbial Physiology and Genetics Subcommittee 2 (MBC)
• Project Start: 1976-01-01
• Project End: 1987-01-31
• Budget Start: 1985-02-01
• Budget End: 1987-01-31
• Support Year: 24
• Fiscal Year: 1985

Institution

• Name: University of California Los Angeles
• Type: Schools of Medicine
• DUNS #: 119132785
• City: Los Angeles
• State: CA
• Country: United States
• Zip Code: 90095

Publications

Edwards, L A; Tian, M R; Huber, R E et al. (1988) The use of limited proteolysis to probe interdomain and active site regions of beta-galactosidase (Escherichia coli). J Biol Chem 263:1848-54

Kurihara, T; Fowler, A V; Takahashi, Y (1987) cDNA cloning and amino acid sequence of bovine brain 2',3'-cyclic-nucleotide 3'-phosphodiesterase. J Biol Chem 262:3256-61

Lee, N; Gielow, W; Martin, R et al. (1986) The organization of the araBAD operon of Escherichia coli. Gene 47:231-44

Hediger, M A (1986) High resolution preparative gel electrophoresis of DNA fragments and plasmid DNA using a continuous elution apparatus. Anal Biochem 159:280-6

Hediger, M A; Johnson, D F; Nierlich, D P et al. (1985) DNA sequence of the lactose operon: the lacA gene and the transcriptional termination region. Proc Natl Acad Sci U S A 82:6414-8

Krzych, U; Fowler, A V; Sercarz, E E (1985) Repertoires of T cells directed against a large protein antigen, beta-galactosidase. II. Only certain T helper or T suppressor cells are relevant in particular regulatory interactions. J Exp Med 162:311-23

Fowler, A V; Hediger, M A; Musso, R E et al. (1985) The amino acid sequence of thiogalactoside transacetylase of Escherichia coli. Biochimie 67:101-8