Abstract

This project will continue to evaluate immunocompetent cells involved in host resistance to infection by intracellular bacteria. Listeria monocytogenes infection in the mouse is used as the model system. We are conducting experiments to determine why injection of either Listeria fractions or thioglycollate medium prior to infection with live Listeria decreases the mouse's ability to resist infection. Experiments to confirm this finding in vitro and to evaluate the mechanism are in progress. Another approach is selective propagation of Listeria specific T cells, whose function will be evaluated. Studies on biologic effects with Listeria fractions include their ability to function as mitogens and adjuvants, as antigens, and as activators of macrophages. Activation of macrophages to kill tumor cells is the criterion by which we measure macrophage activation.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI011240-13
Application #
3124926
Study Section
Bacteriology and Mycology Subcommittee 1 (BM)
Project Start
1976-08-01
Project End
1986-07-31
Budget Start
1985-08-01
Budget End
1986-07-31
Support Year
13
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
National Jewish Health
Department
Type
DUNS #
City
Denver
State
CO
Country
United States
Zip Code
80206

  Publications

Fleming, S D; Leenen, P J; Freed, J H et al. (1999) Surface interleukin-10 inhibits listericidal activity by primary macrophages. J Leukoc Biol 66:961-7
Fleming, S D; Campbell, P A (1997) Some macrophages kill Listeria monocytogenes while others do not. Immunol Rev 158:69-77
Sawyer, R T; Drevets, D A; Campbell, P A et al. (1996) Internalin A can mediate phagocytosis of Listeria monocytogenes by mouse macrophage cell lines. J Leukoc Biol 60:603-10
Roark, C E; Vollmer, M K; Campbell, P A et al. (1996) Response of a gamma delta+ T cell receptor invariant subset during bacterial infection. J Immunol 156:2214-20
Drevets, D A; Leenen, P J; Campbell, P A (1996) Complement receptor type 3 mediates phagocytosis and killing of Listeria monocytogenes by a TNF-alpha- and IFN-gamma-stimulated macrophage precursor hybrid. Cell Immunol 169:1-6
Fleming, S D; Campbell, P A (1996) Macrophages have cell surface IL-10 that regulates macrophage bactericidal activity. J Immunol 156:1143-50
Desbarats, J; Freed, J H; Campbell, P A et al. (1996) Fas (CD95) expression and death-mediating function are induced by CD4 cross-linking on CD4+ T cells. Proc Natl Acad Sci U S A 93:11014-8
Drevets, D A; Elliott, A M (1995) Fluorescence labeling of bacteria for studies of intracellular pathogenesis. J Immunol Methods 187:69-79
Drevets, D A; Sawyer, R T; Potter, T A et al. (1995) Listeria monocytogenes infects human endothelial cells by two distinct mechanisms. Infect Immun 63:4268-76
Drevets, D A; Canono, B P; Leenen, P J et al. (1994) Gentamicin kills intracellular Listeria monocytogenes. Infect Immun 62:2222-8

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