Abstract

Treatment of Sendai virus and NDV nucleocapsids with S. aureus protease cleaves polypeptide P into two domains, an acidic, protease-sensitive portion, and a basic, protease-resistant core, but has little to no effect on polypeptides NP and L. Cleavage of NDV polypeptide P resulted in a proportional loss of RNA synthetic activity suggesting that P is involved in the synthesis of viral RNA. Three approaches were used to show that the hemagglutinating and neuraminidase activities of the HN glycoprotein of paramyxoviruses involve two distinct sites. These activities were separated by analyzing a Sendai virus mutant with a ts HN protein and the use of monoclonal antibodies to HN and an analog of N-acetyl-neuraminic acid. The mRNAs of Sendai virus were separated on agarose urea gels and the RNAs responsible for synthesis of P, NP, and M proteins identified. The ts HN protein of mutant ts 271 was shown to be rapidly degraded after synthesis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI011949-12
Application #
3125068
Study Section
Virology Study Section (VR)
Project Start
1978-07-01
Project End
1987-08-31
Budget Start
1986-09-01
Budget End
1987-08-31
Support Year
12
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
St. Jude Children's Research Hospital
Department
Type
DUNS #
067717892
City
Memphis
State
TN
Country
United States
Zip Code
38105

  Publications

Hurwitz, Julia L (2008) Development of recombinant Sendai virus vaccines for prevention of human parainfluenza and respiratory syncytial virus infections. Pediatr Infect Dis J 27:S126-8
Zaitsev, Viatcheslav; von Itzstein, Mark; Groves, Darrin et al. (2004) Second sialic acid binding site in Newcastle disease virus hemagglutinin-neuraminidase: implications for fusion. J Virol 78:3733-41
Connaris, Helen; Takimoto, Toru; Russell, Rupert et al. (2002) Probing the sialic acid binding site of the hemagglutinin-neuraminidase of Newcastle disease virus: identification of key amino acids involved in cell binding, catalysis, and fusion. J Virol 76:1816-24
Takimoto, Toru; Taylor, Garry L; Connaris, Helen C et al. (2002) Role of the hemagglutinin-neuraminidase protein in the mechanism of paramyxovirus-cell membrane fusion. J Virol 76:13028-33
Bousse, Tatiana; Matrosovich, Tatyana; Portner, Allen et al. (2002) The long noncoding region of the human parainfluenza virus type 1 f gene contributes to the read-through transcription at the m-f gene junction. J Virol 76:8244-51
Takimoto, T; Murti, K G; Bousse, T et al. (2001) Role of matrix and fusion proteins in budding of Sendai virus. J Virol 75:11384-91
Coronel, E C; Takimoto, T; Murti, K G et al. (2001) Nucleocapsid incorporation into parainfluenza virus is regulated by specific interaction with matrix protein. J Virol 75:1117-23
Suzuki, T; Portner, A; Scroggs, R A et al. (2001) Receptor specificities of human respiroviruses. J Virol 75:4604-13
Bousse, T; Takimoto, T; Matrosovich, T et al. (2001) Two regions of the P protein are required to be active with the L protein for human parainfluenza virus type 1 RNA polymerase activity. Virology 283:306-14
Takimoto, T; Taylor, G L; Crennell, S J et al. (2000) Crystallization of Newcastle disease virus hemagglutinin-neuraminidase glycoprotein. Virology 270:208-14

Showing the most recent 10 out of 48 publications