Abstract

The long range objective of this study is to gain insight into the mechanisms of pathogenesis of Chlamydiae. A current hypothesis is that there are special phylogenetic structure(s) responsible for (1) attachment/entry of susceptible cells, (2) initiation and maintenance of intracellular survival in both professional and non-professional phagocytic cells, and (3) immediate cytotoxicity.
One specific aim of this proposal is to determine if the virulence factor(s) responsible for these biological events are located specifically on the elementary body envelope. The basic approach examines the interaction of isolated, purified radiolabeled elementary body envelopes, alone or coated onto latex spheres, with macrophages and mouse L-cells. A second specific aim is to expand our studies of the in vitro interaction of Chlamydiae with professional phagocyte cells and look at both the in vitro and in vivo effects of these intracellular pathogens on various cells of the immune response. Mitogen induced blastogenic transformation of lymphocytes is being used as the initial index of differences between cells from Chlamydiae infected mice versus cells from L-cell infected controls.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI013446-08
Application #
3125442
Study Section
Bacteriology and Mycology Subcommittee 1 (BM)
Project Start
1979-01-01
Project End
1987-07-31
Budget Start
1986-08-01
Budget End
1987-07-31
Support Year
8
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
University of North Carolina Chapel Hill
Department
Type
Schools of Medicine
DUNS #
078861598
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599

  Publications

Rank, Roger G; Whittimore, Judy; Bowlin, Anne K et al. (2011) In vivo ultrastructural analysis of the intimate relationship between polymorphonuclear leukocytes and the chlamydial developmental cycle. Infect Immun 79:3291-301
Rank, Roger G; Lacy, H Marie; Goodwin, Anna et al. (2010) Host chemokine and cytokine response in the endocervix within the first developmental cycle of Chlamydia muridarum. Infect Immun 78:536-44
Wyrick, Priscilla B (2010) Chlamydia trachomatis persistence in vitro: an overview. J Infect Dis 201 Suppl 2:S88-95
Rank, Roger G; Whittimore, Judy; Bowlin, Anne K et al. (2008) Chlamydiae and polymorphonuclear leukocytes: unlikely allies in the spread of chlamydial infection. FEMS Immunol Med Microbiol 54:104-13
Dessus-Babus, Sophie; Moore, Cheryl G; Whittimore, Judy D et al. (2008) Comparison of Chlamydia trachomatis serovar L2 growth in polarized genital epithelial cells grown in three-dimensional culture with non-polarized cells. Microbes Infect 10:563-70
Giles, David K; Wyrick, Priscilla B (2008) Trafficking of chlamydial antigens to the endoplasmic reticulum of infected epithelial cells. Microbes Infect 10:1494-503
Betts, H J; Twiggs, L E; Sal, M S et al. (2008) Bioinformatic and biochemical evidence for the identification of the type III secretion system needle protein of Chlamydia trachomatis. J Bacteriol 190:1680-90
Guseva, Natalia V; Dessus-Babus, Sophie; Moore, Cheryl G et al. (2007) Differences in Chlamydia trachomatis serovar E growth rate in polarized endometrial and endocervical epithelial cells grown in three-dimensional culture. Infect Immun 75:553-64
Giles, David K; Whittimore, Judy D; LaRue, Richard W et al. (2006) Ultrastructural analysis of chlamydial antigen-containing vesicles everting from the Chlamydia trachomatis inclusion. Microbes Infect 8:1579-91
Guseva, Natalia V; Dessus-Babus, Sophie C; Whittimore, Judy D et al. (2005) Characterization of estrogen-responsive epithelial cell lines and their infectivity by genital Chlamydia trachomatis. Microbes Infect 7:1469-81

Showing the most recent 10 out of 46 publications