Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
2R01AI013718-20
Application #
2060016
Study Section
Virology Study Section (VR)
Project Start
1977-09-01
Project End
2001-07-31
Budget Start
1996-08-01
Budget End
1997-07-31
Support Year
20
Fiscal Year
1996
Total Cost
Indirect Cost
Name
Johns Hopkins University
Department
Pharmacology
Type
Schools of Medicine
DUNS #
045911138
City
Baltimore
State
MD
Country
United States
Zip Code
21218
Tullman, Jennifer A; Harmon, Mary-Elizabeth; Delannoy, Michael et al. (2014) Recovery of an HMWP/hmwBP (pUL48/pUL47) complex from virions of human cytomegalovirus: subunit interactions, oligomer composition, and deubiquitylase activity. J Virol 88:8256-67
Fernandes, Steve M; Brignole, Edward J; Taori, Kanchan et al. (2011) Cytomegalovirus capsid protease: biological substrates are cleaved more efficiently by full-length enzyme (pUL80a) than by the catalytic domain (assemblin). J Virol 85:3526-34
Margulies, Barry J; Gibson, Wade (2007) The chemokine receptor homologue encoded by US27 of human cytomegalovirus is heavily glycosylated and is present in infected human foreskin fibroblasts and enveloped virus particles. Virus Res 123:57-71
Loveland, Amy N; Nguyen, Nang L; Brignole, Edward J et al. (2007) The amino-conserved domain of human cytomegalovirus UL80a proteins is required for key interactions during early stages of capsid formation and virus production. J Virol 81:620-8
Wang, Jianlei; Loveland, Amy N; Kattenhorn, Lisa M et al. (2006) High-molecular-weight protein (pUL48) of human cytomegalovirus is a competent deubiquitinating protease: mutant viruses altered in its active-site cysteine or histidine are viable. J Virol 80:6003-12
McCartney, Stephen A; Brignole, Edward J; Kolegraff, Keli N et al. (2005) Chemical rescue of I-site cleavage in living cells and in vitro discriminates between the cytomegalovirus protease, assemblin, and its precursor, pUL80a. J Biol Chem 280:33206-12
Loveland, Amy N; Chan, Chee-Kai; Brignole, Edward J et al. (2005) Cleavage of human cytomegalovirus protease pUL80a at internal and cryptic sites is not essential but enhances infectivity. J Virol 79:12961-8
Chan, Chee-Kai; Brignole, Edward J; Gibson, Wade (2002) Cytomegalovirus assemblin (pUL80a): cleavage at internal site not essential for virus growth; proteinase absent from virions. J Virol 76:8667-74
Baxter, M K; Gibson, W (2001) Cytomegalovirus basic phosphoprotein (pUL32) binds to capsids in vitro through its amino one-third. J Virol 75:6865-73
Plafker, S M; Woods, A S; Gibson, W (1999) Phosphorylation of simian cytomegalovirus assembly protein precursor (pAPNG.5) and proteinase precursor (pAPNG1): multiple attachment sites identified, including two adjacent serines in a casein kinase II consensus sequence. J Virol 73:9053-62

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