Abstract

The overall objective of this project is to further characterize the underlying mechanisms and significance of inflammatory cell responses in the sites of human allergic reactions. The overall approach will utilize techniques adapted by us that permit: 1) sequential measurements of mediator release and inflammatory cell responses in the skin reactions to pollen antigens; 2) sequential measurement of allergic serum chemotactic factors and other mediators after antigen inhalation challenge.
Specific aims i nclude determination: 1) Whether a single antigen challenge in the skin of sensitive subjects leads to a single burst or continued mediator release and how this relates to sequential inflammatory cell responses. 2) Whether qualitatively different mediator release patterns occur in immediate and delayed responses to intradermal or inhaled antigen and how this relates to clinical manifestations of the subjects. 3) The effects of the local allergic environment on subsequent mediator release and inflammatory cell function. 4) The source and biologic effects of neutrophil chemotactic activity we found to be increased during antigen-induced bronchospasm. 5) The effects of immunotherapy on such responses. Findings in this project may provide a basis for more effective treatment in human allergic diseases.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI014332-11
Application #
3125692
Study Section
(SSS)
Project Start
1977-09-01
Project End
1988-08-31
Budget Start
1987-09-01
Budget End
1988-08-31
Support Year
11
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
University of Pennsylvania
Department
Type
Schools of Medicine
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104

  Publications

Zweiman, B; Parrott, C M; Graif, Y et al. (2000) Quantitative comparison of cytokine mRNA and inflammatory responses in cutaneous late phase allergic reactions. Cytokine 12:1065-75
Zweiman, B; von Allmen, C (1999) Comparative effects of antilactoferrin antibodies and tumor necrosis factor on neutrophil adherence to matrix proteins. Clin Diagn Lab Immunol 6:364-8
Lessin, S R; Benoit, B M; Li, G et al. (1999) Quantitative analysis of T-cell receptor beta variable-gene usage in cutaneous late-phase reactions: implications for T-lymphocyte recruitment in cutaneous inflammation. Clin Diagn Lab Immunol 6:85-8
Zweiman, B; von Allmen, C; Schwartz, L B et al. (1998) Patterns of inflammatory responses following rechallenge of skin late-phase allergic reaction sites. J Allergy Clin Immunol 102:94-8
Zweiman, B; Moskovitz, A R; von Allmen, C (1998) Comparison of inflammatory events during developing immunoglobulin E-mediated late-phase reactions and delayed-hypersensitivity reactions. Clin Diagn Lab Immunol 5:574-7
Irani, A M; Huang, C; Xia, H Z et al. (1998) Immunohistochemical detection of human basophils in late-phase skin reactions. J Allergy Clin Immunol 101:354-62
Zweiman, B; Atkins, P C; Moskovitz, A et al. (1997) Cellular inflammatory responses during immediate, developing, and established late-phase allergic cutaneous reactions: effects of cetirizine. J Allergy Clin Immunol 100:341-7
Atkins, P C; Zweiman, B; Moskovitz, A et al. (1997) Cellular inflammatory responses and mediator release during early developing late-phase allergic cutaneous inflammatory responses: effects of cetirizine. J Allergy Clin Immunol 99:806-11
Zweiman, B; Getsy, J; Kalenian, M et al. (1997) Nasal airway changes assessed by acoustic rhinometry and mediator release during immediate and late reactions to allergen challenge. J Allergy Clin Immunol 100:624-31
Xia, H Z; Zweiman, B; Schwartz, L B (1997) Levels of tryptase, chymase, and Fc epsilon RI alpha messenger RNA in human skin are unchanged after IgE-dependent stimulation of cutaneous mast cells in vivo. J Allergy Clin Immunol 99:224-6

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