Abstract

Acquired defense mechanisms against the intestinal dwelling nematode, Trichinella spiralis, will be analyzed in rats and mice with a view to defining the mechanism of rapid expulsion and genetic factors that influence the development and expression of host resistance to infection. Two stimuli are required for the full expression of rapid expulsion in rats. One of these stimuli, provided by adult worms, seems to lack immunological specificity; however, more rigourous evidence is needed to substantiate this notion. The possibility that the above stimulus is related to irritation (inflammation) of the intestinal wall will be examined in experiments using various inhibitors of inflammation, such as salicylates, adrenal corticosteroids, indomethacin, and the factor in cobra venom which cleaves C3. Genetic studies of the rapid expulsion response will be pursued in mice. It has been shown that the capacity to express rapid expulsion is inherited as a dominant characteristic. Back cross experiments are planned to test the proposition that responsiveness is transferred by a gene that lies within or is closely linked to the H-2 complex. If this should prove to be the case, the gene would be mapped in experiments using congenic mice and recombinant strains.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI014490-08
Application #
3125763
Study Section
Tropical Medicine and Parasitology Study Section (TMP)
Project Start
1978-07-01
Project End
1987-08-31
Budget Start
1985-09-01
Budget End
1986-08-31
Support Year
8
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Cornell University
Department
Type
Schools of Veterinary Medicine
DUNS #
City
Ithaca
State
NY
Country
United States
Zip Code
14850

  Publications

Thrasher, S M; Scalfone, L K; Holowka, D et al. (2013) In vitro modelling of rat mucosal mast cell function in Trichinella spiralis infection. Parasite Immunol 35:21-31
Scalfone, Lisa K; Nel, Hendrik J; Gagliardo, Lucille F et al. (2013) Participation of MyD88 and interleukin-33 as innate drivers of Th2 immunity to Trichinella spiralis. Infect Immun 81:1354-63
Blum, L K; Mohanan, S; Fabre, M V et al. (2013) Intestinal infection with Trichinella spiralis induces distinct, regional immune responses. Vet Parasitol 194:101-5
Douglas, Diana B; Beiting, Daniel P; Loftus, John P et al. (2010) Combinatorial effects of interleukin 10 and interleukin 4 determine the progression of hepatic inflammation following murine enteric parasitic infection. Hepatology 51:2162-71
Cwiklinski, Krystyna; Meskill, Diana; Robinson, Mark W et al. (2009) Cloning and analysis of a Trichinella pseudospiralis muscle larva secreted serine protease gene. Vet Parasitol 159:268-71
Fabre, M V; Beiting, D P; Bliss, S K et al. (2009) Immunity to Trichinella spiralis muscle infection. Vet Parasitol 159:245-8
Blum, Lisa K; Thrasher, Seana M; Gagliardo, Lucille F et al. (2009) Expulsion of secondary Trichinella spiralis infection in rats occurs independently of mucosal mast cell release of mast cell protease II. J Immunol 183:5816-22
Fabre, Valeria; Beiting, Daniel P; Bliss, Susan K et al. (2009) Eosinophil deficiency compromises parasite survival in chronic nematode infection. J Immunol 182:1577-83
Wong, Tracie; Hildebrandt, Marie A; Thrasher, Seana M et al. (2007) Divergent metabolic adaptations to intestinal parasitic nematode infection in mice susceptible or resistant to obesity. Gastroenterology 133:1979-88
Beiting, Daniel P; Gagliardo, Lucille F; Hesse, Matthias et al. (2007) Coordinated control of immunity to muscle stage Trichinella spiralis by IL-10, regulatory T cells, and TGF-beta. J Immunol 178:1039-47

Showing the most recent 10 out of 32 publications