Abstract

The long term objective is to understand the regulation of juvenile hormone (JH) synthesis by the corpora allata (CA). The system studied is the female reproductive cycle of the viviparous cockroach, Diploptera punctata, in which egg development is precisely controlled by cyclic changes in production of JH by the CA. This serves as a model system for understanding the regulation of the CA, an important step in developing methods to limit insect reproduction.
The specific aims are to demonstrate how the brain and the ovary act on the CA to alternately stimulate and inhibit the production of JH. The inhibitory and stimulatory factors from these organs will be isolated and their source and mode of action characterized. The cellular and subcellular organization and biochemistry of the CA will be correlated with changes in JH production by the glands during the reproductive cycle. JH synthesis will be measured by a direct short term in vitro radiochemical assay following treatment with extracts of nervous tissue and ovaries. Extracts will be purified with HPLC. The source of the active factors will be demonstrated by cytochemical and immunocytochemical methods. Changes in cellular and subcellular organization will be sought in electron microscopical analyses including autoradiography and stereological measurements.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI015230-10
Application #
3126063
Study Section
Tropical Medicine and Parasitology Study Section (TMP)
Project Start
1979-05-01
Project End
1990-04-30
Budget Start
1989-05-01
Budget End
1990-04-30
Support Year
10
Fiscal Year
1989
Total Cost
Indirect Cost

  Institution

Name
University of Iowa
Department
Type
Schools of Arts and Sciences
DUNS #
041294109
City
Iowa City
State
IA
Country
United States
Zip Code
52242

  Publications

Elliott, Karen L; Woodhead, Andrea P; Stay, Barbara (2006) A stage-specific ovarian factor with stable stimulation of juvenile hormone synthesis in corpora allata of the cockroach Diploptera punctata. J Insect Physiol 52:929-35
Woodhead, A P; Thompson, M E; Chan, K K et al. (2003) Allatostatin in ovaries, oviducts, and young embryos in the cockroach Diploptera punctata. J Insect Physiol 49:1103-14
Rudolph, P H; Stay, B (1997) Cockroach allatostatin-like immunoreactivity in the central nervous system of the freshwater snails Bulinus globosus (Planorbidae) and Stagnicola elodes (Lymnaeidae). Gen Comp Endocrinol 106:241-50
Stay, B; Fairbairn, S; Yu, C G (1996) Role of allatostatins in the regulation of juvenile hormone synthesis. Arch Insect Biochem Physiol 32:287-97
Lange, A B; Chan, K K; Stay, B (1993) Effect of allatostatin and proctolin on antennal pulsatile organ and hindgut muscle in the cockroach, Diploptera punctata. Arch Insect Biochem Physiol 24:79-92
Johnson, G D; Stay, B; Chan, K K (1993) Structure-activity relationships in corpora allata of the cockroach Diploptera punctata: roles of mating and the ovary. Cell Tissue Res 274:279-93
Woodhead, A P; Stoltzman, C A; Stay, B (1992) Allatostatins in the nerves of the antennal pulsatile organ muscle of the cockroach Diploptera punctata. Arch Insect Biochem Physiol 20:253-63
Stay, B; Chan, K K; Woodhead, A P (1992) Allatostatin-immunoreactive neurons projecting to the corpora allata of adult Diploptera punctata. Cell Tissue Res 270:15-23
Stay, B; Woodhead, A P (1990) Response of male corpora allata to ovarian stimulation in the cockroach, Diploptera punctata. Gen Comp Endocrinol 77:127-35
Rankin, S M; Stay, B (1985) Ovarian inhibition of juvenile hormone synthesis in the viviparous cockroach, Diploptera punctata. Gen Comp Endocrinol 59:230-7

Showing the most recent 10 out of 11 publications