I. Overall Goals And Research Strategy This research is aimed at understanding the molecular mechanisms which regulate gene expression in mammalian cells. Two groups of genes in the mouse: those responsible for immunoglobulin production and a set of genes that encode some of the structural proteins of the ribosomes are being studied. The immunoglobulin genes belong to the class of so-called """"""""luxury genes,"""""""" which are expressed only in highly specialized tissues, in this case the B-lymphocyte. These genes are particularly interesting because their expression changes dramatically during the course of lymphocyte maturation, both in a qualitative and quantitative sense. The ribosomal (r) protein genes are categorized as """"""""housekeeping genes."""""""" They are expressed in all types of tissue and are coordinately regulated according to the cells need for new ribosomes. Recombinant DNA methodology is used to isolate genes of interest and to produce appropriately modified derivatives of them. Gene organization and expression are examined by a variety of techniques, including DNA and RNA blots, nucleotide sequence analysis, transcriptional run-on and S1 nuclease protection assays, and appropriate transfection and transformation experiments with purified gene fragments. The knowledge gained by these studies should help establish some of the molecular bases for cellular differentiation and growth regulation.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI017330-10
Application #
3127149
Study Section
Allergy and Immunology Study Section (ALY)
Project Start
1981-01-01
Project End
1990-12-31
Budget Start
1990-01-01
Budget End
1990-12-31
Support Year
10
Fiscal Year
1990
Total Cost
Indirect Cost
Name
Institute for Cancer Research
Department
Type
DUNS #
872612445
City
Philadelphia
State
PA
Country
United States
Zip Code
19111
Genuario, R R; Perry, R P (1996) The GA-binding protein can serve as both an activator and repressor of ribosomal protein gene transcription. J Biol Chem 271:4388-95
Safrany, G; Perry, R P (1995) The relative contributions of various transcription factors to the overall promoter strength of the mouse ribosomal protein L30 gene. Eur J Biochem 230:1066-72
Stokes, D G; Perry, R P (1995) DNA-binding and chromatin localization properties of CHD1. Mol Cell Biol 15:2745-53
Safrany, G; Perry, R P (1993) Transcription factor RFX1 helps control the promoter of the mouse ribosomal protein-encoding gene rpL30 by binding to its alpha element. Gene 132:279-83
Safrany, G; Perry, R P (1993) Characterization of the mouse gene that encodes the delta/YY1/NF-E1/UCRBP transcription factor. Proc Natl Acad Sci U S A 90:5559-63
Delmas, V; Stokes, D G; Perry, R P (1993) A mammalian DNA-binding protein that contains a chromodomain and an SNF2/SWI2-like helicase domain. Proc Natl Acad Sci U S A 90:2414-8
Genuario, R R; Kelley, D E; Perry, R P (1993) Comparative utilization of transcription factor GABP by the promoters of ribosomal protein genes rpL30 and rpL32. Gene Expr 3:279-88
Chung, S; Perry, R P (1993) The importance of downstream delta-factor binding elements for the activity of the rpL32 promoter. Nucleic Acids Res 21:3301-8
Chung, S; Perry, R P (1991) Cell-free transcription of a mouse ribosomal-protein-encoding gene: the effects of promoter mutations. Gene 100:173-80
Hariharan, N; Kelley, D E; Perry, R P (1991) Delta, a transcription factor that binds to downstream elements in several polymerase II promoters, is a functionally versatile zinc finger protein. Proc Natl Acad Sci U S A 88:9799-803

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