Abstract

Mast cells and eosinophils are closely associated in allergic reactions. Eosinophil peroxidase (EPO) when combined with hydrogen peroxide (H202) and a halide forms a strong oxidative system capable of initiating mast cell degranulation. EPO also binds firmly to the secreted mast cell granules (MCG) to form a complex with augmented peroxidatic activity. Objectives of this research are 1) to study the formation and biological properties of the MCG-EPO complex; 2) to study the formation and inactivation of leukotrienes by phagocytes; 3) to study the induction of mast cell secretion by agents released by granulocytes; 4) to study the interaction of mast cell and eosinophil products in the killing of the schistosomula of Schistosoma mansoni and 5) to study the degradation of mast cell heparin by granulocyte products. The overall aim is to study the interaction of mast cells and eosinophils and the role of this interaction in the manifestations of allergic diseases and in the host defense against parasites.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
2R01AI017758-07
Application #
3127430
Study Section
Immunological Sciences Study Section (IMS)
Project Start
1981-04-01
Project End
1993-05-31
Budget Start
1988-06-01
Budget End
1989-05-31
Support Year
7
Fiscal Year
1988
Total Cost
Indirect Cost

  Institution

Name
University of Washington
Department
Type
Schools of Medicine
DUNS #
135646524
City
Seattle
State
WA
Country
United States
Zip Code
98195

  Publications

Henderson Jr, W R; Chi, E Y (1998) The importance of leukotrienes in mast cell-mediated Toxoplasma gondii cytotoxicity. J Infect Dis 177:1437-43
Henderson Jr, W R; Chi, E Y; Albert, R K et al. (1997) Blockade of CD49d (alpha4 integrin) on intrapulmonary but not circulating leukocytes inhibits airway inflammation and hyperresponsiveness in a mouse model of asthma. J Clin Invest 100:3083-92
Henderson Jr, W R; Lewis, D B; Albert, R K et al. (1996) The importance of leukotrienes in airway inflammation in a mouse model of asthma. J Exp Med 184:1483-94
Klebanoff, S J; Coombs, R W (1996) Virucidal effect of stimulated eosinophils on human immunodeficiency virus type 1. AIDS Res Hum Retroviruses 12:25-9
Glover, S; de Carvalho, M S; Bayburt, T et al. (1995) Translocation of the 85-kDa phospholipase A2 from cytosol to the nuclear envelope in rat basophilic leukemia cells stimulated with calcium ionophore or IgE/antigen. J Biol Chem 270:15359-67
McBride, D E; Koenig, J Q; Luchtel, D L et al. (1994) Inflammatory effects of ozone in the upper airways of subjects with asthma. Am J Respir Crit Care Med 149:1192-7
Henderson Jr, W R (1994) The role of leukotrienes in inflammation. Ann Intern Med 121:684-97
Henderson Jr, W R (1994) Role of leukotrienes in asthma. Ann Allergy 72:272-8
Yong, E C; Chi, E Y; Henderson Jr, W R (1994) Toxoplasma gondii alters eicosanoid release by human mononuclear phagocytes: role of leukotrienes in interferon gamma-induced antitoxoplasma activity. J Exp Med 180:1637-48
Chan, S C; Kim, J W; Henderson Jr, W R et al. (1993) Altered prostaglandin E2 regulation of cytokine production in atopic dermatitis. J Immunol 151:3345-52

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