Abstract

The long-range goal of this program is to define the immunobiology of mononuclear phagocy te (MNP) ontogeny. The first phase of this program will focus on the hypothesis that macrophage functional heterogeneity may, in part, be due to the existence of multiple macrophage cell lineages. We will test this hypothesis using murine Ia+ bone marrow-(BM) derived MNP which appear to arise from a progenitor distinct from that yielding the Ia-BM MNP. In addition to determining phenotype stability and associating selected immunological functions with surface phenotype, plans are proposed to characterize established cell lines of MNP.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
2R01AI017979-04
Application #
3127580
Study Section
Experimental Immunology Study Section (EI)
Project Start
1982-07-01
Project End
1988-08-31
Budget Start
1985-09-30
Budget End
1986-08-31
Support Year
4
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
St. Jude Children's Research Hospital
Department
Type
DUNS #
067717892
City
Memphis
State
TN
Country
United States
Zip Code
38105

  Publications

Walker, W S (1999) Separate precursor cells for macrophages and microglia in mouse brain: immunophenotypic and immunoregulatory properties of the progeny. J Neuroimmunol 94:127-33
Havenith, C E; Askew, D; Walker, W S (1998) Mouse resident microglia: isolation and characterization of immunoregulatory properties with naive CD4+ and CD8+ T-cells. Glia 22:348-59
Nguyen, V T; Walker, W S; Benveniste, E N (1998) Post-transcriptional inhibition of CD40 gene expression in microglia by transforming growth factor-beta. Eur J Immunol 28:2537-48
Walker, W S; Castrucci, M R; Sangster, M Y et al. (1997) HEL-Flu: an influenza virus containing the hen egg lysozyme epitope recognized by CD4+ T cells from mice transgenic for an alphabeta TCR. J Immunol 159:2563-6
Askew, D; Gatewood, J; Olivas, E et al. (1995) A subset of splenic macrophages process and present native antigen to naive antigen-specific CD4+ T-cells from mice transgenic for an alpha beta T-cell receptor. Cell Immunol 166:62-70
Olivas, E; Chen, B B; Walker, W S (1995) Use of the Pannell-Milstein roller bottle apparatus to produce high concentrations of the CSF-1, the mouse macrophage growth factor. J Immunol Methods 182:73-9
McCormack, J M; Leenen, P J; Walker, W S (1993) Macrophage progenitors from mouse bone marrow and spleen differ in their expression of the Ly-6C differentiation antigen. J Immunol 151:6389-98
McCormack, J M; Askew, D; Walker, W S (1993) Alloantigen presentation by individual clones of mouse splenic macrophages. Selective expression of IL-1 alpha in response to CD8+ T cell-derived IFN-gamma defines the alloantigen-presenting phenotype. J Immunol 151:5218-27
Moore, S C; McCormack, J M; Armendariz, E et al. (1992) Phenotypes and alloantigen-presenting activity of individual clones of microglia derived from the mouse brain. J Neuroimmunol 41:203-14
McCormack, J M; Moore, S C; Gatewood, J W et al. (1992) Mouse splenic macrophage cell lines with different antigen-presenting activities for CD4+ helper T cell subsets and allogeneic CD8+ T cells. Cell Immunol 145:359-71

Showing the most recent 10 out of 24 publications