Abstract

This proposal concerns the genetic analysis of influenza A, B and C viruses. 1.) We will clone and sequence several NP and M genes of influenza A viruses, the HA, NP, NA, M and NS genes of influenza B viruses and the corresponding genes of influenza C viruses. 2.) We will attempt to express cloned influenza virus genes in eucaryotic cells using SV40 and retrovirus vector systems. These experiments are designed to study the function of individual genes and regions of the genes. Cells constitutively expressing influenza virus polypeptides should, via complementation, permit the isolation of specific viral mutants. Furthermore, we will attempt to establish a system which permits the rescue of RNA from cloned DNA into virus particles. 3.) We will continue our studies on the characterization of influenza virus polypeptides, including analysis of the M2 polypeptide of influenza A viruses. We will also explore the possibility that open reading frames on the negative strand of some influenza virus NS genes direct the synthesis of a viral polypeptide in vivo. We will also establish a genetic map for influenza C viruses.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI018998-04
Application #
3128417
Study Section
Experimental Virology Study Section (EVR)
Project Start
1982-05-01
Project End
1987-04-30
Budget Start
1985-05-01
Budget End
1986-04-30
Support Year
4
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Mount Sinai School of Medicine
Department
Type
Schools of Medicine
DUNS #
City
New York
State
NY
Country
United States
Zip Code
10029

  Publications

Park, Man-Seong; Steel, John; Garcia-Sastre, Adolfo et al. (2006) Engineered viral vaccine constructs with dual specificity: avian influenza and Newcastle disease. Proc Natl Acad Sci U S A 103:8203-8
Lowen, Anice C; Mubareka, Samira; Tumpey, Terrence M et al. (2006) The guinea pig as a transmission model for human influenza viruses. Proc Natl Acad Sci U S A 103:9988-92
O'Neill, R E; Talon, J; Palese, P (1998) The influenza virus NEP (NS2 protein) mediates the nuclear export of viral ribonucleoproteins. EMBO J 17:288-96
O'Neill, R E; Jaskunas, R; Blobel, G et al. (1995) Nuclear import of influenza virus RNA can be mediated by viral nucleoprotein and transport factors required for protein import. J Biol Chem 270:22701-4
Piccone, M E; Fernandez-Sesma, A; Palese, P (1993) Mutational analysis of the influenza virus vRNA promoter. Virus Res 28:99-112
Li, X; Palese, P (1992) Mutational analysis of the promoter required for influenza virus virion RNA synthesis. J Virol 66:4331-8
Luo, G; Palese, P (1992) Genetic analysis of influenza virus. Curr Opin Genet Dev 2:77-81
Luo, G; Bergmann, M; Garcia-Sastre, A et al. (1992) Mechanism of attenuation of a chimeric influenza A/B transfectant virus. J Virol 66:4679-85
Bergmann, M; Garcia-Sastre, A; Palese, P (1992) Transfection-mediated recombination of influenza A virus. J Virol 66:7576-80
Lin, D A; Roychoudhury, S; Palese, P et al. (1991) Evolutionary relatedness of the predicted gene product of RNA segment 2 of the tick-borne Dhori virus and the PB1 polymerase gene of influenza viruses. Virology 182:1-7

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